Evidence for genetic differentiation and recent succession of benthic foraminifers: effect of ecological factors

• Project/Area Number: 17K05696
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Stratigraphy/Paleontology
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: TSUCHIYA Masashi 国立研究開発法人海洋研究開発機構, 地球環境部門(海洋生物環境影響研究センター), グループリーダー代理 (00435835)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 層位・古生物学 / 系統進化 / 海洋生態 / 古環境変遷 / 底生有孔虫 / 同位体生態 / 分子系統学 / 共生

Outline of Final Research Achievements

In this study, we demonstrated that the genetic differentiation mechanism of the rocky shore benthic foraminifera (Elphidium crispum, Pararotalia nipponica) living along the coast of Japan is not only influenced by geographical isolation such as the closure of the Sea of Japan, but also by endobitic microalgae and host ecological factors from the perspectives of (1) molecular phylogeography, (2) ecology, and (3) stable isotope ecology. Although the two species have similar sexual reproductive behavior, they demonstrated different genetic differentiation patterns. We found that ecological factors such as endobiotic microalgal differences and its environmental response, and differences in the host foraminiferal food preferences are likely to contribute to the formation of local population and the genetic differentiation of the host foraminifera.

Academic Significance and Societal Importance of the Research Achievements

海洋酸性化や温暖化など海洋環境変化は、基礎生産の一翼を担う有孔虫などの多様性や生物量にも影響を及ぼすため、個体や集団だけではなく、海洋生態系構造を変化させうる。その結果、海洋生態系の恩恵を享受する人間活動においても、影響を与える可能性が高い。環境変化に対して有孔虫がどのように微小生息空間に適応し集団を形成するのか、どのように遺伝的分化するのかを解明することで、将来起こりうる環境変動に対する石灰化生物の応答を評価できる。有孔虫を利用した海洋酸性化実験では、宿主や共生藻の種ごとに殻の溶解度が異なる。宿主の環境応答や代謝だけではなく、集団の形成にも共生藻類との相互作用の解明が必要であることを示す。

Research Products

• [Int'l Joint Research] リール大学(フランス)
• [Int'l Joint Research] ウルビノ大学(イタリア)
• [Int'l Joint Research] ヘルシンキ大学(フィンランド)
• [Int'l Joint Research] ジュネーブ大学(スイス)
• [Int'l Joint Research] Geomarine Research(ニュージーランド)
• [Int'l Joint Research] University of Geneva/Ecole Polytechnique Federale de Lausanne/University of Lausanne(スイス)
• [Int'l Joint Research] University of Helsinki(フィンランド)
• [Int'l Joint Research] Universite d'Angers(フランス)
• [Int'l Joint Research] Woods Hole Oceanographic Institution(米国)
• [Int'l Joint Research] University of Bremen(ドイツ)
• [Int'l Joint Research] ウッズホール海洋研究所(米国)
• [Int'l Joint Research] アンジェ大学(フランス)
• [Int'l Joint Research] スイス連邦工科大学ローザンヌ校/ローザンヌ大学/ジュネーブ大学(スイス)
• [Int'l Joint Research] ヘルシンキ大学(フィンランド)
• [Journal Article] Characterization of microplastics on filter substrates based on hyperspectral imaging: Laboratory assessments (2020)
  • Author(s): Zhu Chunmao、Kanaya Yugo、Nakajima Ryota、Tsuchiya Masashi、Nomaki Hidetaka、Kitahashi Tomo、Fujikura Katsunori
  • Journal Title: Environmental Pollution Volume: 263 Pages: 114296-114296
  • DOI: 10.1016/j.envpol.2020.114296
  • Peer Reviewed / Open Access
• [Journal Article] Sediment sampling with a core sampler equipped with aluminum tubes and an onboard processing protocol to avoid plastic contamination (2019)
  • Author(s): Tsuchiya Masashi、Nomaki Hidetaka、Kitahashi Tomo、Nakajima Ryota、Fujikura Katsunori
  • Journal Title: MethodsX Volume: 6 Pages: 2662-2668
  • DOI: 10.1016/j.mex.2019.10.027
  • Peer Reviewed / Open Access
• [Journal Article] Combined Molecular and Morphological Taxonomy of the Beccarii/T3 Group of the Foraminiferal Genus Ammonia (2019)
  • Author(s): Hayward Bruce W.、Holzmann Maria.、Tsuchiya Masashi
  • Journal Title: Journal of Foraminiferal Research Volume: 49 Issue: 4 Pages: 367-389
  • DOI: 10.2113/gsjfr.49.4.367
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A new small device made of glass for separating microplastics from marine and freshwater sediments (2019)
  • Author(s): Ryota Nakajima, Masashi Tsuchiya, Dhugal J. Lindsay, Tomo Kitahashi, Katsunori Fujikura and Tomohiko Fukushima
  • Journal Title: PeerJ Volume: 7 Pages: e7915-e7915
  • DOI: 10.7717/peerj.7915
  • Peer Reviewed / Open Access
• [Journal Article] A small, stainless-steel sieve optimized for laboratory beaker-based extraction of microplastics from environmental samples (2019)
  • Author(s): Nakajima R, Lindsay DJ, Tsuchiya M, Matsui R, Kitahashi T, Fujikura K, Fukushima T
  • Journal Title: MethodsX Volume: 6 Pages: 1677-1682
  • DOI: 10.1016/j.mex.2019.07.012
  • Peer Reviewed / Open Access
• [Journal Article] Taming chlorophylls allowed eukaryotes to prosper on the oxygenated Earth. (2019)
  • Author(s): Kashiyama Y, Yokoyama A, Shiratori T, Bachy C, Gutierrez-Rodriguez A, Not F, Hess S, Wang M, Chen M, Gong Y, Seto K, Kagami M, Hamamoto Y, Honda D, Umetani T, Shihongi A, Kayama M, Matsuda T, Taira J, Yabuki A, Tsuchiya M, Hirakawa Y, Kawaguchi A, Nomura M, Nakamura A, Namba N, Matsumoto M, Tanaka T, Yoshino T et al.
  • Journal Title: The ISME journal Volume: - Issue: 8 Pages: 1-12
  • DOI: 10.1038/s41396-019-0377-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Compound-specific isotope analysis of benthic foraminifer amino acids suggests microhabitat variability in rocky-shore environments. (2018)
  • Author(s): Tsuchiya M, Chikaraishi Y, Nomaki H, Sasaki Y, Tame A, Uematsu K, Ohkouchi N
  • Journal Title: Ecology and Evolution Volume: 00 Issue: 16 Pages: 1-16
  • DOI: 10.1002/ece3.4358
  • Peer Reviewed / Open Access
• [Journal Article] A new analytical method for determination of the nitrogen isotopic composition of methionine: Its application to aquatic ecosystems with mixed resources. (2018)
  • Author(s): Ishikawa NF, Chikaraishi Y, Takano Y, Sasaki Y, Takizawa Y, Tsuchiya M, Tayasu I, Nagata T, Ohkouchi N
  • Journal Title: Limnology & Oceanography: Methods Volume: 16 Issue: 9 Pages: 607-620
  • DOI: 10.1002/lom3.10272
  • Peer Reviewed / Open Access
• [Journal Article] A new method for acquiring images of meiobenthic images using the FlowCAM. (2018)
  • Author(s): Kitahashi T, Watanabe H, Tsuchiya M, Yamamoto Hideyuki, Yamamoto Hiroyuki
  • Journal Title: Methods X Volume: 5 Pages: 1330-1335
  • Peer Reviewed
• [Journal Article] Ultrastructural observations on prokaryotic associates of benthic foraminifera: Food, mutualistic symbionts, or parasites? (2018)
  • Author(s): Bernhard Joan M.、Tsuchiya Masashi、Nomaki Hidetaka
  • Journal Title: Marine Micropaleontology Volume: 138 Pages: 33-45
  • DOI: 10.1016/j.marmicro.2017.09.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ultrastructure and distribution of kleptoplasts in benthic foraminifera from shallow-water (photic) habitats (2018)
  • Author(s): Jauffrais Thierry、LeKieffre Charlotte、Koho Karoliina A.、Tsuchiya Masashi、Schweizer Magali、Bernhard Joan M.、Meibom Anders、Geslin Emmanuelle
  • Journal Title: Marine Micropaleontology Volume: 138 Pages: 46-62
  • DOI: 10.1016/j.marmicro.2017.10.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Syringammina limosa sp. nov., the first xenophyophore from the deep Sea of Okhotsk: morphological and genetic description (2017)
  • Author(s): Voltski Ivan、Weiner Agnes K.M.、Tsuchiya Masashi、Kitazato Hiroshi
  • Journal Title: Deep Sea Research Part II: Topical Studies in Oceanography Volume: in press Pages: 32-46
  • DOI: 10.1016/j.dsr2.2017.12.001
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Elphidum batialisの分子系統学的位置と深海への適応・集団の形成機構 (2020)
  • Author(s): 土屋正史、長谷川四郎
  • Organizer: 2019年度 微化石リファレンスセンター（MRC）研究集会
• [Presentation] 膠着質有孔虫を用いたマイクロプラスチック汚染の実態把握 (2020)
  • Author(s): 土屋正史
  • Organizer: 2019年度 微化石リファレンスセンター（MRC）研究集会
• [Presentation] ナイルレッド染色したマイクロプラスチックの自動検出法開発 (2020)
  • Author(s): 土屋正史、北橋 倫、平 陽介、斎藤仁志、中嶋亮太、Dhugal J. Lindsay、藤倉克則、福島朋彦
  • Organizer: 第54回日本水環境学会（2019年度）
• [Presentation] ハイパースペクトルカメラを用いたマイクロプラスチックの材質自動判別の試み (2020)
  • Author(s): 北橋 倫、中嶋亮太、野牧秀隆、土屋正史、山口壮二朗、朱 春茂、金谷有剛、Dhugal Lindsay、千葉早苗、藤倉克則
  • Organizer: 第54回日本水環境学会（2019年度）
• [Presentation] Ecological factors affecting the formation and persistence of genetic populations of rocky-shore benthic foraminifera (2019)
  • Author(s): Masashi Tsuchiya、Katsunori Fujikura
  • Organizer: TMS 2019, The Micropaleontological Society’s Joint Foraminifera and Nanofossil Meeting
  • Int'l Joint Research
• [Presentation] Microplastic as a "micro"indicator for Anthropocene: understanding the actual condition of microplastic pollution by using agglutinated foraminifera (2019)
  • Author(s): Masashi Tsuchiya
  • Organizer: TMS 2019, The Micropaleontological Society’s Joint Foraminifera and Nanofossil Meeting
  • Int'l Joint Research
• [Presentation] Metabarcoding reveals putative interactions between sediment bacteria and deep-sea benthic foraminifera (2019)
  • Author(s): Iines Salonen、Panagiota-Myrsini Chronopoulou、Dewi Langlet、Hidetaka Nomaki、Masashi Tsuchiya、KaroliinaKoho K
  • Organizer: TMS 2019, The Micropaleontological Society’s Joint Foraminifera and Nanofossil Meeting
  • Int'l Joint Research
• [Presentation] A new threat for marine environments and foraminifera: micro- and nano- plastics (TMS Workshop) (2019)
  • Author(s): Hidetaka Nomaki、Fabrizio Frontalini、Vincent Bouchet、Dewi Langlet、Masashi Tsuchiya
  • Organizer: TMS 2019, The Micropaleontological Society’s Joint Foraminifera and Nanofossil Meeting
  • Int'l Joint Research
• [Presentation] Variations in 3-D cytoplasmic structures of foraminifera (protistan meiofauna): microfocus X-ray CT and TEM observations (2019)
  • Author(s): Hidetaka Nomaki、Masashi Tsuchiya、Akihiro Tame、Takuya Matsuzaki、Masafumi Murayama
  • Organizer: 17th International Meiofauna Conference
  • Int'l Joint Research
• [Presentation] 人新世の微化石指標：砂質有孔虫を用いたマイクロプラスチック汚染の実態把握 (2019)
  • Author(s): 土屋正史、野牧秀隆、木元克典、中嶋亮太、北橋 倫、川村喜一郎、生田哲朗、小栗一将、千葉早苗、藤倉克則
  • Organizer: 日本古生物学会2019年年会
• [Presentation] 底生有孔虫Chilostomella細胞内の板状構造と推察される役割 (2019)
  • Author(s): 野牧秀隆、Chen Chong、小田加也、土屋正史、松崎琢也、多米晃裕、植松勝之
  • Organizer: 日本古生物学会2019年年会
• [Presentation] 人新世の微化石指標：人為的なマイクロプラスチック化石の検出と変遷 (2019)
  • Author(s): 土屋正史、斎藤めぐみ、辻本 彰、野牧秀隆
  • Organizer: 日本古生物学会2019年年会（夜間小集会）
• [Presentation] 砂質有孔虫を用いたマイクロプラスチック汚染の実態把握 (2019)
  • Author(s): 土屋正史
  • Organizer: 2019年日本ベントス学会・日本プランクトン学会・合同大会（自由集会）
• [Presentation] メイオファウナを用いた環境影響評価手法の検討 (2019)
  • Author(s): 北橋 倫，中村匡聡，西島美由紀，猪又健太郎，土屋正史，渡部裕美，山本啓之
  • Organizer: ブルーアースサイエンス・テク2019
• [Presentation] 海底堆積物からマイクロプラスチックを分離するための新しい器具の考案 (2019)
  • Author(s): 中嶋亮太，土屋正史，北橋 倫，Dhugal Lindsay，藤倉克則，福島朋彦
  • Organizer: 第53回日本水環境学会年会（2018年度）
• [Presentation] Syringammina limosa sp. nov. and other xenophyophores from the north-west Pacific (the Sea of Okhotsk and Kuril-Kamchatka Trench region) (2018)
  • Author(s): Voltski I, Weiner AKM, Tsuchiya M, Kitazato H
  • Organizer: International Symposium on Foraminifera (FORAMS 2018)
  • Int'l Joint Research
• [Presentation] A comprehensive look on the endobiotic community and ecology of deep-sea foraminifera Bolivina subornata. (2018)
  • Author(s): Karoliina A. Koho, Iines Salonen, Hidetaka Nomaki, Myrsini Chronopoulou, Dewi Langlet and Masashi Tsuchiya
  • Organizer: International Symposium on Foraminifera (FORAMS 2018)
  • Int'l Joint Research
• [Presentation] The adaptation potential of benthic foraminifera from extremely warm reefs in the Persian Gulf. (2018)
  • Author(s): Schmidt C, Morard, R, Burt J, Theara G, Vaughan G, Tsuchiya M, Kucera M
  • Organizer: International Symposium on Foraminifera (FORAMS 2018)
  • Int'l Joint Research
• [Presentation] Cellular ultrastructure in benthic foraminifera: general observations and focus on kleptoplasts. (2018)
  • Author(s): LeKieffre C, Jauffrais T, Geslin E, Koho KA, Tsuchiya M, Meibom A, Bernhard J
  • Organizer: International Symposium on Foraminifera (FORAMS 2018)
  • Int'l Joint Research
• [Presentation] Variation in foraminiferal cytoplasm structures and its possible relationships between environmental conditions. (2018)
  • Author(s): Nomaki H, Tsuchiya M, Tame A, Matsuzaki T
  • Organizer: International Symposium on Foraminifera (FORAMS 2018)
  • Int'l Joint Research
• [Presentation] Abyssal ecosystems and biogeochemical cycles revealed by in-situ experiments. (2018)
  • Author(s): Nomaki H, Rastelli E, Amaro T, Tsuchiya M, Seike K, Kitahashi T, Suga H, Miyamoto N, Ogawa NO, Danovaro R, Nunoura T, Ohkouchi N
  • Organizer: 15th Deep Sea Biology Symposium 2018
  • Int'l Joint Research
• [Presentation] Detecting the effect of scientific drilling on meiofauna with the traditional, metagenomic and image-based methods (2018)
  • Author(s): Kitahashi T, Sugime S, Nakamura M, Nishijima M, Inomata K, Tsuchiya M, Watanabe HK, Yamamoto H
  • Organizer: 15th Deep Sea Biology Symposium 2018
  • Int'l Joint Research
• [Presentation] A new small device made of glass for separating microplastics from marine sediments. (2018)
  • Author(s): Nakajima R, Tsuchiya M, Kitahashi T, Lindsay D, Fujikura K, Fukushima T
  • Organizer: The Association for the Sciences of Limnology and Oceanography (ASLO 2018)
  • Int'l Joint Research
• [Presentation] 深海底科学掘削に伴う掘削屑がメイオファウナ群集に与えた影響ーイメージング・フローサイトメーターを用いた結果との比較 (2018)
  • Author(s): 北橋 倫，杉目幸恵，土屋正史，渡部裕美，山本啓之
  • Organizer: 日本地球惑星科学連合2018年大会
• [Presentation] 生態的要因による岩礁地性底生有孔虫の遺伝的集団の遷移と形成 (2018)
  • Author(s): 土屋正史，石渡祐一，吉田尊雄，藤倉克則
  • Organizer: 日本古生物学会2018年年会
• [Presentation] 有孔虫類の分子生物学的研究 (2018)
  • Author(s): 土屋正史
  • Organizer: 日本古生物学会2018年年会
  • Invited
• [Presentation] 掘足類ヤカドツノガイと経卵伝達される細菌の関係 (2018)
  • Author(s): 生田哲朗，多米晃裕，高木善弘，齋藤正輝，力石嘉人，土屋正史，藤倉克則，吉田尊雄
  • Organizer: 第2回日本共生生物学会
• [Presentation] アミノ酸窒素同位体比に基づく栄養段階推定によって明らかになった微小生息空間内の多様な資源利用形態 (2018)
  • Author(s): 土屋正史，力石嘉人，野牧秀隆，佐々木瑶子，多米晃裕，植松勝之，大河内直彦
  • Organizer: 日本古生物学会第167回例会
• [Presentation] 底生有孔虫Globobulimina affinisの細胞立体構造・微細構造の特徴と貧酸素環境への適応戦略 (2018)
  • Author(s): 野牧秀隆，土屋正史，多米晃裕，矢生晋介，松崎琢也，村山雅史
  • Organizer: 日本古生物学会第167回例会
• [Presentation] 有孔虫の起源と進化 (2018)
  • Author(s): 土屋正史
  • Organizer: 2017年度微古生物学リファレンスセンター研究集会
  • Invited
• [Presentation] Elphidiidae科有孔虫の系統関係の解明 (2018)
  • Author(s): 石渡祐一，土屋正史，吉田尊雄，藤倉克則
  • Organizer: 2017年度微古生物学リファレンスセンター研究集会
• [Presentation] 嫌気環境下における底生有孔虫Ammonia sp.の適応機構 (2017)
  • Author(s): 土屋正史，野牧秀隆
  • Organizer: 日本古生物学会2017年年会
• [Presentation] 深海底科学掘削に伴う掘削屑がメイオファウナ群集に与えた影響 (2017)
  • Author(s): 北橋 倫，杉目幸恵，土屋正史
  • Organizer: 2017年日本プランクトン学会・日本ベントス学会合同大会
• [Book] アメーバのはなし (2018)
  • Author(s): 永宗 喜三郎、島野 智之、矢吹 彬憲（「2.8.2自由生活性」「コラム 微化石」を分担執筆）
  • Total Pages: 152
  • Publisher: 朝倉書店
  • ISBN: 9784254171686