| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Pulsed ESR dipolar spectroscopy (PDS) methodology was extended to cases where the high-field approximation breaks down. Equations for the dipolar coupling constant was derived. It was shown on a heme protein that a PDS method called relaxation-induced dipolar modulation enhancement is well-suited to measuring such spectra and that the experimentally obtained dipolar spectra are in full agreement with the derived equations. We introduced a spin labeling strategy based on click chemistry in solution that, in combination with enzymatic ligation, allows highly efficient labeling of complex. Conformations of the preQ1 aptamer and its dynamics were monitored by ESR and PDS in the frozen state. A rapid freezing equipment was successfully constructed, and applied to the reaction of metmyoglobin with sodium azide, which was monitored by ESR.
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