Laser-induced fluorescence and solvent effects of gas-phase isolated ions

• Project/Area Number: 17K05759
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: University of Hyogo
• Principal Investigator: HONMA KENJI 兵庫県立大学, 物質理学研究科, 特任教授(名誉教授) (30150288)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: レーザー誘起蛍光 / イオントラップ / 分散蛍光スペクトル / イオン移動度分析 / 質量分析 / 孤立分子状態イオン / レーザー誘起蛍光スペクトル / 高振動励起状態の発光 / タンパク質のコンフォーメーション変化 / 気相タンパク質のコンフォーメーション変化 / エレクトロスプレーイオン化

Outline of Final Research Achievements

Laser-induced fluorescence and dispersed fluorescence techniques were applied for ions formed by electrospray ionization (ESI) and trapped in collision free condition. Dynamical behavior of electronically excited states of ions could be derived without any perturbation of solvents. Relaxation processes of higher excited states formed by a uv photon were studied for the first time. This research has open a new possibility to study photo-absorption and –emission of gas-phase ions which can be prepared only by the ESI technique. An infrared laser was also applied to heat droplets formed by the ESI technique to study heat induced conformation changes of biological molecular ions such as protein ions.

Academic Significance and Societal Importance of the Research Achievements

エレクトロスプレーイオン化(ESI)法で初めて気相に取りだすことのできるイオン種について、光吸収・放出という電子励起状態の挙動解明に最も一般的な手法を適用し、観測領域を紫外光へ初めて拡張した。その結果、高い電子励起状態から内部転換によって生成した非常に高い振動励起状態からの発光観測に初めて成功した。この手法は、生体高分子の溶液中、あるいは生体中で挙動を観測する主な手法である光吸収・放出の観測を気相中でも実現可能にし、直接比較へ道を開いた。また、ESI液滴に赤外線レーザーを照射して加熱する手法は、高分子の熱誘起相転移の機構を解明する新しい道手法となる可能性がある。

Research Products

• [Int'l Joint Research] Indiana University(米国)
• [Int'l Joint Research] Indiana University(米国)
• [Int'l Joint Research] Indiana University/University of Utah(米国)
• [Journal Article] IR Cavity Ringdown Spectroscopy and Density Functional Theory for Jet-Cooled Pyrrole?Cyclopentanone Binary Clusters: Effect of Pseudorotation on N?H・・・O?C Hydrogen Bonds (2020)
  • Author(s): Matsumoto Yoshiteru、Honma Kenji
  • Journal Title: The Journal of Physical Chemistry A Volume: 124 Issue: 12 Pages: 2436-2448
  • DOI: 10.1021/acs.jpca.0c00794
  • Peer Reviewed
• [Journal Article] Laser-induced- and dispersed-fluorescence studies of rhodamine 590 and 640 ions formed by electrospray ionization: Observation of fluorescence from highly-excited vibrational levels of S1 states. (2018)
  • Author(s): Kenji Honma
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 20 Issue: 42 Pages: 26859-26869
  • DOI: 10.1039/c8cp04067b
  • Peer Reviewed
• [Journal Article] Identification of crystalline structures in jet-cooled acetylene large clusters studied by two-dimensional correlation infrared spectroscopy (2017)
  • Author(s): Matsumoto Yoshiteru、Yoshiura Ryuto、Honma Kenji
  • Journal Title: The Journal of Chemical Physics Volume: 147 Issue: 4 Pages: 044302-044302
  • DOI: 10.1063/1.4994897
  • Peer Reviewed
• [Journal Article] Reaction dynamics of Al+CO2->AlO+CO studied by a crossed-beam velocity map imaging technique. (2017)
  • Author(s): Kenji Honma and Daiki Hirata
  • Journal Title: The Journal of Chemical Physics Volume: １４７ Issue: 1 Pages: 013903-013903
  • DOI: 10.1063/1.4974943
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Melting proteins in microdroplets induced by laser heating (2019)
  • Author(s): HONMA, Kenji; WOODALL, D. W.; CLEMMER, D. E.
  • Organizer: 35th Symposium on Chemical Kinetics and Dynamics
  • Int'l Joint Research
• [Presentation] レーザー照射による液滴中のタンパク質のコンフォーメーション変化 (2019)
  • Author(s): 本間健二, WOODAL, Dan, CLEMMER, David. E.
  • Organizer: 第13回分子科学討論会
• [Presentation] Fluorescence measurement of rhodamine ions under isolated gas-phase condition. (2018)
  • Author(s): HONMA, Kenji
  • Organizer: 34th Symposium on Chemical Kinetics and Dynamics
  • Int'l Joint Research
• [Presentation] 真空中に単離した気相イオンのLIF/DF観測：生体分子観測の可能性 (2018)
  • Author(s): 本間健二
  • Organizer: 第12回分子科学討論会
• [Presentation] Observation of Fluorescence from Highly Excited-Vibrational Levels of S1 States of Gas-Phase Rhodamine Cations. (2018)
  • Author(s): Kenji Honma
  • Organizer: 10th Asian Photochemistry Conference 2018
  • Int'l Joint Research / Invited
• [Presentation] Optical spectroscopy of isolated gas-phase ions (2017)
  • Author(s): HONMA, Kenji; NAGAOKA,Ayaka; TSURUOKA, Maho; MAKI, Wakaba
  • Organizer: 33th Symposium on Chemical Kinetics and Dynamics
  • Int'l Joint Research
• [Presentation] 真空中に単離したイオンのLIF/DF観測 (2017)
  • Author(s): 本間健二
  • Organizer: 第11回分子科学討論会
• [Presentation] 交差分子線ー画像観測法による反応ダイナミクスの解明 (2017)
  • Author(s): 本間健二
  • Organizer: 第11回分子科学討論会
  • Invited
• [Presentation] Optical spectroscopy of isolated gas-phase ions (2017)
  • Author(s): Kenji Honma
  • Organizer: International Symposium on “Diversity of Chemical Reaction Dynamics”
  • Int'l Joint Research / Invited
• [Funded Workshop] International Symposium on “Diversity of Chemical Reaction Dynamics” (2017)