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Fluorescence Detection of Saccharide Based on a Complexation between Aggregate Emission Dye and Lectin

Research Project

Project/Area Number 17K05849
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional solid state chemistry
Research InstitutionKurume National College of Technology

Principal Investigator

Ishi-i Tsutomu  久留米工業高等専門学校, 生物応用化学科, 教授 (60346856)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords発光 / 会合 / ドナー・アクセプター / 糖質 / レクチン / 生体検出 / 生体材料 / 超分子化学 / 光物性 / 糖 / 有機化学
Outline of Final Research Achievements

In this study, we report fluorescence detection of saccharide based on a complexation between aggregate emission dye and lectin. The dye bearing a saccharide moiety, which is fluorescent in the aggregate state, indicates a fluorescence quenching in the monomer state after the complexation with lectin. In contrast, an emission recovery could be achieved by treatment with target saccharide, as a result of the dissociation of the complex between dye and lectin, and the subsequent aggregate formation of dye molecules. The present exchange between aggregate emission and monomer quenching can be applied to the fluorescence detection of target saccharide.

Academic Significance and Societal Importance of the Research Achievements

本研究では、レクチンを用いる糖質蛍光検出に、会合発光というアイデアを付与することで、単純で合理的な戦略を提案している。単純な色素基盤とレクチンとの複合体を用いて、蛍光 OFF-ON 変化で糖質を検出する基礎的な研究内容であるが、レクチン-糖質の組合せに留まらず、抗原―抗体等の様々な生体アフィニティー系での蛍光検出の可能性を秘めた研究でもあり、基礎から応用までの一貫した研究の基盤を確立できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Invited: 3 results) Remarks (2 results)

  • [Journal Article] pH-Responsive Fluorescence Change Based on Dynamic Exchange between Emitting Aggregate and Quenching Monomer in Donor-Acceptor Dyes bearing Carboxylic Groups2018

    • Author(s)
      Tsutomu Ishi-i, Taishi Nakanishi
    • Journal Title

      European Journal of Organic Chemistry

      Volume: 9 Issue: 9 Pages: 1165-1173

    • DOI

      10.1002/ejoc.201800025

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 会合性ドナー・アクセプター型蛍光色素によるレクチン検出システムの構築2019

    • Author(s)
      加藤智紀・古賀早和子・石井努
    • Organizer
      第 60 回化学関連支部合同九州大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 超分子会合による水系長波長発光システムの構築2019

    • Author(s)
      石井努
    • Organizer
      有機合成化学協会中国四国支部パネル討論会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ドナー・アクセプター型蛍光色素のレクチン複合体形成と会合発光特性変化2019

    • Author(s)
      加藤智紀・古賀早和子・石井努
    • Organizer
      日本化学会第 99 春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] ドナー・アクセプター型蛍光色素のレクチン複合体形成による発光特性変化2018

    • Author(s)
      樋口亜也斗・古賀早和子・石井努
    • Organizer
      日本化学会第 98 春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] ドナー・アクセプター共役分子における発光特性の探索2017

    • Author(s)
      石井努
    • Organizer
      先端有機化学講演会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] ドナー・アクセプター型蛍光色素の新規特性を探索する2017

    • Author(s)
      石井努
    • Organizer
      2nd Symposium on New Trends of Nano- or Bio-Materials Design in Supramolecular Chemistry
    • Related Report
      2017 Research-status Report
    • Invited
  • [Remarks] 久留米工業高等専門学校生物応用化学科石井研究室ホームページ

    • URL

      http://www.cc.kurume-nct.ac.jp/~ishi-i/world/index.html

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
  • [Remarks] 久留米工業高等専門学校生物応用化学科石井研究室ホームページ

    • URL

      http://apollo.cc.kurume-nct.ac.jp/~ishi-i/research/research.html

    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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