Development of the nucleic acid sensing probe based on molecule transfer.

• Project/Area Number: 17K05898
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Gifu University
• Principal Investigator: Shibata Aya 岐阜大学, 工学部, 助教 (50462693)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 遺伝子検出 / 芳香族求核置換反応 / バイオ分析

Outline of Final Research Achievements

In this research, we tried development of the nucleic acid sensing probe based on molecule transfer which is triggered by a nucleic aromatic substituted (SNAr) reaction. In addition, the probe was designed to be easily multi-coloring by the click reaction.
We synthesized three probes with different structures and evaluated them. As a result, it was confirmed that molecular transfer due to the SNAr reaction occurs in all the probes. In addition, we found that the NO2-CN type probe is the most desirable.

Academic Significance and Societal Importance of the Research Achievements

生細胞内の遺伝子検出法として標的核酸を鋳型とした化学反応プローブがある。この遺伝子検出法の利点は標的核酸を鋳型とした反応サイクルを回すことで、シグナルを増幅することができる点にある。しかし、これらのプローブの多くは蛍光基質の構造変化をシグナル発生の鍵としているため、使用できる蛍光波長に制限がある。本研究ではこの問題を解決すべく、蛍光分子をポストで修飾可能な化学反応プローブを開発した。