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Post column chiral separation for simultaneous determination of chiral compounds

Research Project

Project/Area Number 17K05905
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Analytical chemistry
Research InstitutionKumamoto University

Principal Investigator

Ohira Shin-Ichi  熊本大学, 大学院先端科学研究部(理), 准教授 (60547826)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords光学分割 / 溶存イオン抽出 / ポストカラム / インライン濃縮 / 電気透析 / アミノ酸 / フェニル乳酸
Outline of Final Research Achievements

Lives can differentiate the chiral compounds. On the other hand, separation of chiral compounds is required high level separation techniques. Acceleration of the study on the function of chiral compounds in lives and environments can be achieved with rapid and reliable quantitation methods. In the present study, ion transfer technology was applied to achieve chiral separation with a single solution path. The developed method can be used as post column separation method and simultaneous quantitation of chiral compounds. The best separation performance was obtained with ion transfer membrane functionalization. Additionally, newly developed cross flow ion transfer device was suitable for post column ion transfer method which can keep the peak resolution.

Academic Significance and Societal Importance of the Research Achievements

光学異性体は,その違いが生体内によって認識されることから,異なる作用を示している。しかしながら,これらの分離は困難であり,製薬,食品などの製造プロセスを煩雑かつ高コストなものにしている。本研究の成果によって,光学異性体の高い分離特性が得られたため,分離に係るコスト面での大幅な改善が見込まれる。また,分析化学への展開により,光学異性体の分析技術が進展することで,生体・環境における動態の解明が進むと期待される。特に,近年,疾病と特定の光学異性体の関係も報告されるようになってきており,新しい医療診断技術への展開も期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (26 results)

All 2020 2019 2018 2017 Other

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results) Remarks (2 results)

  • [Journal Article] Semi-continuous Monitoring of Cr(VI) and Cr(III) during a Soil Extraction Process by Means of an Ion Transfer Device and Graphite Furnace Atomic Absorption Spectroscopy2020

    • Author(s)
      NUGRAHA Willy Cahya、NAGAI Haruka、OHIRA Shin-Ichi、TODA Kei
    • Journal Title

      Analytical Sciences

      Volume: 36 Issue: 5 Pages: 617-620

    • DOI

      10.2116/analsci.19SBN02

    • NAID

      130007840920

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2020-05-10
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Electrodialytic Handling of Radioactive Metal Ions for Preparation of Tracer Reagents2020

    • Author(s)
      Sugo Yumi、Miyachi Ryoma、Maruyama Yo-hei、Ohira Shin-Ichi、Mori Masanobu、Ishioka Noriko S.、Toda Kei
    • Journal Title

      Analytical Chemistry

      Volume: 92 Issue: 22 Pages: 14953-14958

    • DOI

      10.1021/acs.analchem.0c02456

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Miniaturized crossflow ion transfer device for post-column enrichment in ion chromatography2020

    • Author(s)
      Ohira Shin-Ichi、Sakaki Tomomi、Miyachi Ryoma、Otsubo Aoi、Umemoto Asami、Kuwahara Yoshihide、Toda Kei
    • Journal Title

      Talanta

      Volume: 216 Pages: 120989-120989

    • DOI

      10.1016/j.talanta.2020.120989

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
  • [Journal Article] Universal Detection Methods in Ion Chromatography2019

    • Author(s)
      OHIRA Shin-Ichi、TODA Kei
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 68 Issue: 3 Pages: 153-162

    • DOI

      10.2116/bunsekikagaku.68.153

    • NAID

      130007623962

    • ISSN
      0525-1931
    • Year and Date
      2019-03-05
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electrodialytic Matrices Isolation for Determination of Heavy Metals in Soil Extracts by Anodic Stripping Voltammetry2018

    • Author(s)
      OHIRA Shin-Ichi、OKAZAKI Chinatsu、TODA Kei
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 67 Issue: 12 Pages: 761-766

    • DOI

      10.2116/bunsekikagaku.67.761

    • NAID

      130007554783

    • ISSN
      0525-1931
    • Year and Date
      2018-12-05
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Universal HPLC Detector for Hydrophilic Organic Compounds by Means of Total Organic Carbon Detection2018

    • Author(s)
      Ohira Shin-Ichi、Kaneda Kyosuke、Matsuzaki Toru、Mori Shuta、Mori Masanobu、Toda Kei
    • Journal Title

      Analytical Chemistry

      Volume: 90 Issue: 11 Pages: 6461-6467

    • DOI

      10.1021/acs.analchem.7b04849

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Electrodialytic in-line preconcentration for ionic solute analysis2018

    • Author(s)
      Ohira Shin-Ichi、Yamasaki Takayuki、Koda Takumi、Kodama Yuko、Toda Kei
    • Journal Title

      Talanta

      Volume: 180 Pages: 176-181

    • DOI

      10.1016/j.talanta.2017.12.054

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultra-sensitive Trace-Water Optical Sensor with <i>In situ</i>- synthesized Metal&ndash;Organic Framework in Glass Paper2018

    • Author(s)
      Shin-Ichi OHIRA, Nao NAKAMURA, Masaaki ENDO, Yusuke MIKI, Yasuo HIROSE, Kei TODA
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 4 Pages: 495-500

    • DOI

      10.2116/analsci.17P453

    • NAID

      130006682640

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Admittance Scanning for Whole Column Detection2017

    • Author(s)
      Stamos Brian N.、Dasgupta Purnendu K、Ohira Shin-Ichi
    • Journal Title

      Analytical Chemistry

      Volume: 89 Issue: 13 Pages: 7203-7209

    • DOI

      10.1021/acs.analchem.7b01412

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Review: Speciation Analysis of Chromium Oxidation States by Flow Injection Analysis2017

    • Author(s)
      Ohira Shin-Ichi、Nugraha, Willy Chaya、Toda Kei
    • Journal Title

      Journal of Flow Injection Analysis

      Volume: 34 Pages: 5-10

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Miniaturized cross flow electrodialytic device for flow injection analysis and post-column enhancement on an ion chromatography2019

    • Author(s)
      Shin-Ichi Ohira
    • Organizer
      International symposium on miniaturized systems for chemical separation and analysis
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] pHによる有機酸のユニバーサル検出2019

    • Author(s)
      大平慎一,佐藤裕香,戸田 敬
    • Organizer
      第36回イオンクロマトグラフィー討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] 技術賞受賞講演:電場と膜透過を利用した溶存イオン抽出による前処理・濃縮・分離技術の開発2019

    • Author(s)
      大平慎一
    • Organizer
      第36回イオンクロマトグラフィー討論会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 電気透析による放射性金属イオンの抽出とカウンターイオンの置換2019

    • Author(s)
      大平慎一,宮地凌,丸山洋平,須郷由美,石岡典子,森 勝伸,戸田 敬
    • Organizer
      第38回溶媒抽出討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] pH Linear Gradient Generation by Miniaturized Electro-dialysis Device for Chromatographic Separation2018

    • Author(s)
      Bhakti Dwi Yoga, Shin-Ichi Ohira, Kei Toda
    • Organizer
      Asianalysis XIV
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 電気透析による緩衝溶液の生成とpHのフィードバック制御2018

    • Author(s)
      大平慎一,Bhakti Dwi Yoga,森 秀太,伊藤貴啓,戸田 敬
    • Organizer
      第78回分析化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] 標準物質フリーなHPLCの検出システム2018

    • Author(s)
      大平慎一
    • Organizer
      第31回バイオメディカル分析科学シンポジウム
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 電場と膜透過による光学分割の基礎検討2018

    • Author(s)
      堀内一希,大平慎一,戸田 敬
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Sample pretreatment and separation of ionic solutes by means of electrodialysis2018

    • Author(s)
      Shin-Ichi Ohira
    • Organizer
      3rd International Symposium on Green Technology for Value Chains (GreenVC 2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 標準物質フリーなユニバーサル検出器とその応用2017

    • Author(s)
      大平慎一,金田恭介,戸田 敬
    • Organizer
      第77回分析化学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] Quantification System without Standard Materials for High Performance Liquid Chromatography (HPLC)2017

    • Author(s)
      大平慎一
    • Organizer
      第23回日本化学会九州支部・韓国化学会釜山支部合同セミナー
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 電場による泳動と膜透過による分離手法の展開2017

    • Author(s)
      大平慎一,Willy Cahya Nugraha,戸田 敬
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Calibration free detection system for HPLC2017

    • Author(s)
      Shin-Ichi Ohira, Kyosuke Kaneda, Kei Toda
    • Organizer
      Asia/ CJK symposium on analytical science 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ダイナミックな相間移動による  前処理・分離・検出2017

    • Author(s)
      大平慎一
    • Organizer
      徳島大学薬学部 第5回Brightシンポジウム
    • Related Report
      2017 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.sci.kumamoto-u.ac.jp/~ohira/index.html

    • Related Report
      2018 Research-status Report
  • [Remarks]

    • URL

      http://www.sci.kumamoto-u.ac.jp/~ohira/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2022-12-28  

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