| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To apply a photo-receptor membrane protein bacteriorhodopsin (bR) to a new optical functional device, we developed methods in protein immobilization with artificial life environments. The immobilization methods that bR can function as if in the solution environments was searched and the immobilized bR samples were made. The immobilization samples by hydrogels in which can retain huge water content showed that bR can keep its native functionality even in the solid samples, by an assessment by a time-resolved absorption spectroscopy. We studied about a mechanism for the purple membrane stacking in the hydrogel which was discovered in our previous research. We revealed that both the periodic structure of the purple membranes in solution emerged as a result of balance between electrostatic repulsive force and attractive depletion force and immobilization with a porous gel network structure.
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