| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We developed spherical nucleic acids having a core structure of self-immolative polymer that can undergo disassembly through a self-immolative fragmentation. A poly(carbamate)（PC）derivative composed of 4-aminobenzyl alcohol as a repeat unit was treated with carbonyldiimidazole, followed by conjugation with DNA on a solid support to give a DNA-PC conjugate. Subsequent cleavage from the solid support and dialysis against water resulted in a self-assembly of DNA-PC conjugate with a narrow unimodal size distribution in DLS measurements, indicating formation of nanostructure covered with a dense DNA shell in aqueous solution. The nanostructure thus obtained may undergo domino-like disassembly of PC that can release 4-aminobenzyl alcohol at several triggers. More importantly, this methodology leads to the creation of carrier-free DDS by replacing each unit in the PC with a pharmaceutical drug having a 4-aminobenzyl alcohol substitution.
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