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Substitution of the energy-accepting membranous protein with amphiphilic synthetic pigments in photosynthetic supramolecular complexes

Research Project

Project/Area Number 17K05940
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionKindai University

Principal Investigator

Saga Yoshitaka  近畿大学, 理工学部, 教授 (60411576)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords光合成 / クロロゾーム / クロロフィル / バクテリオクロロフィル / 励起エネルギー移動 / エネルギー移動 / 緑色光合成細菌 / 光捕集
Outline of Final Research Achievements

Understanding of the energy transfer mechanisms among supramolecules in photosynthetic systems is important in the research area of photobiology and developments of artificial light-conversion systems. From these viewpoints, we foucused on major light-harvesting supramolecular systems called chlorosomes from green photosynthetic bacteria. In chlorosomes, excitation energy captured by bacteriochlorophyll self-aggregates inside is transferred to the pigment-binding membranous protein on the surface with a high efficiency. We successfully substituted the energy-accepting membranous protein with amphiphilic synthetic pigments in chlorosomes. We analayzed excitation energy transfer from natural bacteriochlorophyll self-aggregates inside the chlorosome to synthetic amphiphilic pigments that were localized close to the chlorosomal membrane.

Academic Significance and Societal Importance of the Research Achievements

光合成超分子の間の緻密な連携とそれに基づいた高機能発現の機構解明は、光合成をシステムとして理解するうえで重要である。本研究での、異種の色素複合超分子で構成される系のエネルギードナーとアクセプターの関係性の積極的な改変は、このような課題への新たなアプローチとして学術的意義がある。また、分子生物学的方法論では困難であった膜タンパク質部分の改変を、両親媒性合成分子への置換によって克服できる可能性を本研究で示したことも学術的に有意義であるともに、生体膜工学としての応用展開も期待でき社会的意義があると思われる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (35 results)

All 2020 2019 2018 2017

All Journal Article (10 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (25 results) (of which Int'l Joint Research: 8 results,  Invited: 1 results)

  • [Journal Article] Reconstitution of 3-Acetyl Chlorophyll a into Light-Harvesting Complex 2 from the Purple Photosynthetic Bacterium Phaeospirillum molischianum2020

    • Author(s)
      Saga Yoshitaka、Yamashita Madoka、Imanishi Michie、Kimura Yukihiro、Masaoka Yuto、Hidaka Tsubasa、Nagasawa Yutaka
    • Journal Title

      ACS Omega

      Volume: 5 Issue: 12 Pages: 6817-6825

    • DOI

      10.1021/acsomega.0c00152

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural effects on epimerization of bacteriochlorophyll a and chlorophyll a revealed using 3-acetyl chlorophyll a2020

    • Author(s)
      Saga Yoshitaka、Nakagawa Shiori
    • Journal Title

      Journal of Porphyrins and Phthalocyanines

      Volume: 24 Issue: 04 Pages: 499-504

    • DOI

      10.1142/s1088424620500054

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In situ Conversion of Chlorophyll b Reconstituted into Photosynthetic Protein LH22019

    • Author(s)
      Saga Yoshitaka、Yamashita Madoka、Nakagawa Shiori
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 10 Pages: 1270-1273

    • DOI

      10.1246/cl.190545

    • NAID

      130007724091

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH22019

    • Author(s)
      Saga Yoshitaka、Kawano Kiyoshiro、Otsuka Yuji、Imanishi Michie、Kimura Yukihiro、Matsui Sayaka、Asakawa Hitoshi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 3636-3636

    • DOI

      10.1038/s41598-019-40082-y

    • NAID

      120006599151

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconstitution of Chlorophyll d into the Bacterial Photosynthetic Light-harvesting Protein LH22018

    • Author(s)
      Saga Yoshitaka、Yamashita Madoka、Imanishi Michie、Kimura Yukihiro
    • Journal Title

      Chemistry Letters

      Volume: 47 Issue: 8 Pages: 1071-1074

    • DOI

      10.1246/cl.180483

    • NAID

      130007430950

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of 3-Acetyl Chlorophyll a and 3-Acetyl Protochlorophyll a Accommodated in the B800 Binding Sites in Photosynthetic Light-Harvesting Complex 2 in the Puple Photosynthetic Bacterium Rhodoblastus acidophilus2018

    • Author(s)
      Saga Yoshitaka、Miyagi Kanji
    • Journal Title

      Phptpchemistry and Photobiology

      Volume: 印刷中 Issue: 4 Pages: 698-704

    • DOI

      10.1111/php.12919

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective Removal of B800 Bacteriochlorophyll a from Light-Harvesting Complex 2 of the Purple Photosynthetic Bacterium Phaeospirillum molischianum2018

    • Author(s)
      Saga Yoshitaka、Hirota Keiya、Matsui Sayaka、Asakawa Hitoshi、Ishikita Hiroshi、Saito Keisuke
    • Journal Title

      Biochemistry

      Volume: 57 Issue: 21 Pages: 3075-3083

    • DOI

      10.1021/acs.biochem.8b00259

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Amphiphilic zinc bacteriochlorophyll a derivatives that function as artificial energy acceptors in photosynthetic antenna complexes chlorosomes of the green sulfur photosynthetic bacterium Chlorobaculum limnaeum2018

    • Author(s)
      Saga Yoshitaka、Takahashi Naoya、Miyatake Tomohiro、Tamiaki Hitoshi
    • Journal Title

      Journal of Photochemistry and Photobiology A: Chemistry

      Volume: 353 Pages: 612-617

    • DOI

      10.1016/j.jphotochem.2017.08.001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Insertion of chlorophyll a derivatives into the binding sites of B800 bacteriochlorophyll a in light-harvesting complex 2 from the purple photosynthetic bacterium Rhodoblastus acidophilus2018

    • Author(s)
      Saga Yoshitaka、Amari Kenta、Miyagi Kanji
    • Journal Title

      Journal of Photochemistry and Photobiology A: Chemistry

      Volume: 353 Pages: 591-596

    • DOI

      10.1016/j.jphotochem.2017.07.039

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 光合成タンパク質からの色素脱離を基盤とした機能化2017

    • Author(s)
      佐賀佳央
    • Journal Title

      光化学

      Volume: 48 Pages: 166-169

    • NAID

      40021423257

    • Related Report
      2017 Research-status Report
  • [Presentation] 緑色光合成細菌の光捕集アンテナ複合体のエネルギー受容膜タンパク質の人工分子による置換・修飾2019

    • Author(s)
      佐賀佳央、山下隼人、小林巧
    • Organizer
      2019年光化学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 3-アセチルクロロフィルaを利用したバクテリオクロロフィルaとクロロフィルaのエピマー化の反応性に対する構造要因の解明2019

    • Author(s)
      中川支央里、佐賀佳央
    • Organizer
      第27回光合成セミナー
    • Related Report
      2019 Annual Research Report
  • [Presentation] 両親媒性クロロフィル誘導体を用いた緑色硫黄光合成細菌のクロロゾームの光捕集機能改変2019

    • Author(s)
      佐賀佳央、山下隼人
    • Organizer
      第10回日本光合成学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] バクテリオクロロフィルaとクロロフィルaのエピマー化反応に対する分子構造の影響の解析2019

    • Author(s)
      中川支央里、佐賀佳央
    • Organizer
      第10回日本光合成学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Comparison of Epimerization Kinetics among Bacteriochlorophyll a, Chlorophyll a, and 3-Acetyl Chlorophyll a2019

    • Author(s)
      Nakagawa Shiori, Saga Yoshitaka
    • Organizer
      15th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 佐賀佳央、山下隼人2019

    • Author(s)
      緑色光合成細菌の光捕集超分子複合体・クロロゾームと合成亜鉛クロロフィル誘導体の複合化
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 紅色光合成細菌Phaeospirillum molischianumのLH2タンパク質のB800部位への3-アセチルクロロフィルaの再構成2019

    • Author(s)
      山下眞花、佐賀佳央
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 紅色光合成細菌のLH2タンパク質のB800部位へ導入したクロロフィル色素の分光特性2018

    • Author(s)
      山下眞花、佐賀佳央
    • Organizer
      2018年光化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] LH2タンパク質のB800バクテリオクロロフィルaのクロリン環色素への置換2018

    • Author(s)
      山下眞花・大塚悠史・川野聖志朗・今西三千絵・木村行宏・佐賀佳央
    • Organizer
      第26回光合成セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] Preparation of Isomers of Bacteriochlorophyll a Derivatives for Investigation of Their Structural Effects on Reconstitution into LH2 Protein from the Purple Photosynthetic Bacterium Rhodoblastus acidophilus2018

    • Author(s)
      Y. Saga, S. Nakagawa, K. Miyagi
    • Organizer
      14th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reconstitution of Chlorophyll Derivatives into the LH2 Protein from the Purple Photosynthetic Bacterium Rhodoblastus acidophilus2018

    • Author(s)
      M. Yamashita, Y. Saga
    • Organizer
      14th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Removal of B800 Bacteriochlorophyll a from Structure-Determined Light-Harvesting Proteins 2 in Purple Photosynthetic Bacteria2018

    • Author(s)
      佐賀佳央、廣田圭耶
    • Organizer
      Biophysical Society 62th Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 紅色光合成細菌の光捕集タンパク質LH2に存在するB800バクテリオクロロフィルaの部分的脱離と物性解析2018

    • Author(s)
      佐賀佳央、福田吉隆、宮城貫志
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 紅色光合成細菌の光捕集タンパク質LH2へのクロロフィルdの再構成2018

    • Author(s)
      佐賀佳央、山下眞花
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Regulation of the Occupancy of B800 Bacteriochlorophyll a in Light-harvesting Complex 2 from a Purple Photosynthetic Bacterium Rhodoblastus acidophilus2017

    • Author(s)
      佐賀佳央、福田吉隆
    • Organizer
      13th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparison of the Qy Absorption Bands of Bacteriochlorophyll a in LH2 Proteins Derived from Three Purple Photosynthetic Bacteria, Rhodoblastus acidophilus, Phaeospirillum molischianum, and Rhodobacter sphaeroides2017

    • Author(s)
      佐賀佳央、廣田圭耶
    • Organizer
      13th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Spectroscopic and Structural features of the Light-Harvesting Complexes 2 in Purple Photosynthetic Bacteria Induced by Removal of B800 Bacteriochlorophyll a2017

    • Author(s)
      佐賀佳央、廣田圭耶、宮城貫志、淺川雅
    • Organizer
      13th International Conference on Tetrapyrrole Photoreceptors of Photosynthetic Organisms
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Hybridization of Amphiphilic Zinc Chlorophyll Derivatives with Light-harvesting Complexes Chlorosomes in Green Sulfur Photosynthetic Bacteria2017

    • Author(s)
      山下隼人、高橋直哉、原田二朗、民秋均、佐賀佳央
    • Organizer
      The Second International Symposium on Biofunctional Chemistry
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 緑色硫黄光合成細菌の脂質生合成反応を利用したクロロゾームの糖脂質の脂肪酸部位の細胞内改変2017

    • Author(s)
      佐賀佳央、吉田望見、山田翔大、溝口正、民秋均
    • Organizer
      第8回日本光合成学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 紅色光合成細菌の光捕集タンパク質LH2からの色素脱離によって生成した空隙群へのクロロフィル誘導体の挿入2017

    • Author(s)
      佐賀佳央、甘利健太、宮城貫志、廣田圭耶、淺川雅
    • Organizer
      第15回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 紅色光合成細菌の光捕集タンパク質LH2の色素置換と分光特性解析2017

    • Author(s)
      佐賀佳央、宮城貫志、甘利健太、山下眞花、廣田圭耶、淺川雅
    • Organizer
      第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 紅色光合成細菌の光捕集タンパク質LH2へのクロロフィル類の再構成:バクテリオクロリン環からクロリン環への変換の影響2017

    • Author(s)
      宮城貫志、佐賀佳央
    • Organizer
      第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 構造解明されている2種類の紅色光合成細菌のLH2タンパク質からのB800バクテリオクロロフィルaの脱離挙動解析2017

    • Author(s)
      佐賀佳央、廣田圭耶、土居倖実
    • Organizer
      日本生物物理学会第55回年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 紅色光合成細菌の光捕集タンパク質LH2からの部分的に色素欠損した色素ペプチド複合体の調製2017

    • Author(s)
      佐賀佳央、福田吉隆
    • Organizer
      第54回ペプチド討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] 光合成タンパク質の色素集積構造を利用した機能開拓2017

    • Author(s)
      佐賀佳央
    • Organizer
      第11回日本化学会東海支部若手研究者フォーラム
    • Related Report
      2017 Research-status Report
    • Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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