Effect of chemical environment changes in cell on DNA structures and functions
| Project/Area Number |
17K05941
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Konan University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 細胞内環境 / 構造変化 / 非二重らせん構造 / 転写変異 / がん遺伝子 / 定量的解析 / DNA構造 / 擬似細胞内環境 / センシング / がん / 転写 / 核酸 / 核酸非標準構造 / 溶液環境 |
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| Outline of Final Research Achievements |
The relationship between the G-quadruplex formation and disease onset has received attention because the formation of G-quadruplexes induces mutations in various biological reactions. Here, we focused on the intracellular environment peculiar to diseases such as cancer and neurodegenerative diseases, and analyzed the effect of environmental changes in the cell on the structure and function of DNA. Furthermore, based on the findings, we have developed an artificial nucleic acid that stabilizes the G-quadruplex in order to suppress biological reactions in diseased cells.
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| Academic Significance and Societal Importance of the Research Achievements |
DNAの非二重らせん構造である四重らせん構造が形成されると、転写・翻訳反応など、生体内の重要な反応の変異が誘発さるため、四重らせん構造の形成と疾患発症機構との関連性が注目されている。本研究では、DNAは遺伝子の情報を保持するという役割をもつだけでなく、DNAの“構造”を介して生体反応を制御するという“機能”をもつことを試験管内、細胞内の実験において示すことができた。
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Report
(4 results)
Research Products
(67 results)
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[Journal Article] An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex2018
Author(s)
K. Kawauchi, W. Sugimoto, T. Yasui, K. Murata, K. Itoh, K. Takagi, T. Tsuruoka, K. Akamatsu, H. Tateishi-Karimata, N. Sugimoto, and D. Miyoshi
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Journal Title
Nat. Commum
Volume: 9
Issue: 1
Pages: 2271-2271
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Unexpected Position-Dependent Effects of Ribose G-Quartets in G-Quadruplexes2017
Author(s)
Zhou Jun、Amrane Samir、Rosu Frederic、Salgado Gilmar F.、Bian Yunqiang、Tateishi-Karimata Hisae、Largy Eric、Korkut Dursun Nizam、Bourdoncle Anne、Miyoshi Daisuke、Zhang Jian、Ju Huangxian、Wang Wei、Sugimoto Naoki、Gabelica Valerie、Mergny Jean-Louis
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Journal Title
Journal of the American Chemical Society
Volume: 139
Issue: 23
Pages: 7768-7779
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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