Influence of topography of vessel endothelium on rolling characteristics of neutrophils

• Project/Area Number: 17K06140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fluid engineering
• Research Institution: Kindai University (2018-2019); Tohoku University (2017)
• Principal Investigator: Shirai Atsushi 近畿大学, 工学部, 教授 (20302226)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: HL-60 / Rolling / Surface topography / Pressing force / Endothelium / P-selectin / 好中球 / ローリング / 血管内皮細胞 / 傾斜遠心顕微鏡

Outline of Final Research Achievements

The aim of this study is elucidation of correlative effects of pressing of neutrophils onto endothelium by collision with erythrocytes and surface topography of the endothelium on the neutrophils’ rolling behavior. In this study, the surface topography was modeled by staggered short hexagonal poles to make the model endothelium by PDMS. Behaviors of HL-60 cells on regular and elongated hexagonal patterns of the model endothelium were compared under a variety of pressing force using the inclined centrifuge microscope system. Consequently, it can be said that probability of neutrophils' passage through periphery of endothelial cells does not dependent on the endothelial cell shape but on the pressing force. Elongation of the endothelial cells, however, less affects deceleration of the rolling due to their slim shape.

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた結果は，好中球のローリング挙動には，従来から知られている血管内皮細胞表面のセレクチンと血球のリガンドとの結合・離脱に加えて，赤血球との衝突や血管内皮表面の幾何形状も重要な影響を与えることを示している．本結果から，生体内において，炎症が発生すると血管が拡張して赤血球の軸集中が起こるので，これによって血管壁に押しつけられた好中球は，セレクチンが局在する血管内皮細胞の辺縁部を選択的に通ることで効率的にセレクチンと結合すると考えられる．そして，これによって血球は更に活性化され，血管外遊走の次のステップへ短時間に移ることができると推察される．

Research Products

• [Journal Article] Differentiation of neutrophil-like HL-60 cells strongly impacts their rolling on surfaces with various adhesive properties under a pressing force. (2018)
  • Author(s): Shirai A, Sugiyama Y, Rieu JP.
  • Journal Title: Technology and Health Care Volume: 26 Issue: 1 Pages: 93-108
  • DOI: 10.3233/thc-171052
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 微小血管における白血球流動のモデリング (2019)
  • Author(s): 白井 敦
  • Organizer: 近畿大学工学部研究公開フォーラム2019
  • Invited
• [Presentation] Modeling of Alveolar Microcirculatino for Passage of Neutrophils (2018)
  • Author(s): Atsushi Shirai
  • Organizer: The 15th International Conference on Flow Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Rolling characteristics of neutrophils on PDMS surface mimicking the endothelial topography: Influence of pressing force on cell trajectories (2018)
  • Author(s): Shirai, A., Rieu, J.-P.
  • Organizer: ELyT Workshop 2018
  • Int'l Joint Research
• [Presentation] 血管内皮表面を模擬したPDMS基板上におけるHL-60細胞の挙動解析（P-セレクチンの塗布による影響） (2017)
  • Author(s): 荒井俊貴, 白井敦, Jean-Paul RIEU
  • Organizer: 日本機械学会第30回バイオエンジニアリング講演会
• [Presentation] Rolling experiment of HL-60 cells on patterned PDMS substrate coated by P-selectin (2017)
  • Author(s): Arai, T., Shirai, A., Rieu, J.-P.
  • Organizer: 14th International Conference on Flow Dynamics (ICFD2017)
  • Int'l Joint Research