High throughput evaluation method for the bacterial biofilm stabiliry in wastewater treatment using a sensor

• Project/Area Number: 17K06627
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: Kobayashi Takuro 国立研究開発法人国立環境研究所, 資源循環・廃棄物研究センター, 主任研究員 (10583172)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 生物膜 / センサー / 嫌気性処理 / カチオン / 高塩濃度 / メタン発酵 / 産業排水 / 排水処理 / 微生物 / バイオセンサー

Outline of Final Research Achievements

Anaerobic wastewater treatment is an established method for energy-saving and reducing waste production. Retention of attached microorganisms is a key to overcome the slow growth rate of anaerobic microorganisms. In this study, we proposed an effective evaluation method to detect slight change caused by biofilm formation using a sensor. Using the proposed method, we elucidated the phenomenon regarding reduced biofilm formation in the presence of cationic substances.

Academic Significance and Societal Importance of the Research Achievements

嫌気性処理は応用範囲が近年大きく拡大し、様々な業種で採用されるようになった。生物膜法は最も安価に実現可能な効率的処理方法である。近年は排水に含有される成分に起因する生物膜の形成阻害により本法の適用が制限されるケースが認められるようになった。本研究では、センサーを使った微小な質量変化から、短期間で生物膜の発達を評価する方法を構築した。これを利用し、阻害の原因物質やその濃度、さらに阻害軽減のための方策について、実験室内で検討することが可能であることが示された。

Research Products

• [Journal Article] An Integrated Anaerobic System for On-Site Treatment of Wastewater From Food Waste Disposer (2020)
  • Author(s): Yong Hu, Chen Shi, Takuro Kobayashi, Kai-Qin Xu
  • Journal Title: Environmental Science and Pollution Research volume Volume: 27 Issue: 15 Pages: 17587-17595
  • DOI: 10.1007/s11356-019-06651-4
  • Peer Reviewed
• [Journal Article] Impact of cationic substances on biofilm formation from sieved fine particles of anaerobic granular sludge at high salinity (2018)
  • Author(s): Takuro Kobayashi, Hu Yong, Kai-Qin Xu
  • Journal Title: Bioresource Technology Volume: 257 Pages: 69-75
  • DOI: 10.1016/j.biortech.2018.02.078
  • Peer Reviewed
• [Presentation] 農業残渣の炭化物を活用したメタン生成グラニュールの発達促進 (2020)
  • Author(s): 胡勇, Chen SHI, 馬海元, 小林拓朗, 徐開欽
  • Organizer: SATテクノロジー・ショーケース2020
• [Presentation] Biofilm formation enhancement in anaerobic treatment of high salinity wastewater: Effect of ferric polymer addition (2019)
  • Author(s): Yong Hu, Chen Shi, Haiyuan Ma, Takuro Kobayashi, Kai-Qin Xu
  • Organizer: The 4th International Conference on Alternative Fuels, Energy and Environment (ICAFEE 2019)
  • Int'l Joint Research
• [Presentation] 嫌気性生物膜形成に及ぼす金属カチオン等の相互作用による影響 (2018)
  • Author(s): 小林拓朗、徐開欽
  • Organizer: 第21回日本水環境学会シンポジウム
• [Presentation] Monitoring and characterization of biofilm development in anaerobic treatment systems under different conditions using quartz crystal sensor (2017)
  • Author(s): Kobayashi T., Xu K-Q.
  • Organizer: The 2nd International Conference on Alternative Fuels and Energy (ICAFE’17)
  • Int'l Joint Research
• [Presentation] Monitoring and characterization of biofilm formation in anaerobic treatment systems under different conditions using quartz crystal sensor (2017)
  • Author(s): Kobayashi T., Xu K-Q.
  • Organizer: The 2017 International Environmental Engineering Conference & Annual Meeting of the Korean Society of Environmental Engineers
  • Int'l Joint Research / Invited