Fundamental study for sensitivity to exopolymeric substances of graphene in organisms and its applicability to sensors
Project/Area Number |
17K06826
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Composite materials/Surface and interface engineering
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Research Institution | Suzuka National College of Technology |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
平井 信充 鈴鹿工業高等専門学校, その他部局等, 教授 (50294020)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | biofilm / EPS / impedance / capacitance / graphene / spin resonance / biofiom sensor / two layer graphene / バイオフィルム / 細胞外重合物質 / グラフェン / バイオフィルムセンサー / 二層グラフェン / インピーダンス / スピン共鳴 / 有機ポリマー / センサー / 多糖 / タンパク質 / グラファイト / ラマン分光 / クリスタルバイオレット / 大腸菌 / 表皮ブドウ球菌 / 感染 / 電気化学特性 |
Outline of Final Research Achievements |
Graphene was produced on various materials, using different processes and Biofilms(BF) was formed on them artificially. Then we found the graphene has high sensitivity to BF formation. Therefore, we produced two layer graphene on copper substrates and formed biofilms on them artificially, using biofilm reactors. And the biofilms' impedance was measured. As a result, we observed that the capacitance of materials surfaces showed peaks at some characteristic frequencies, when biofilms formed. The capacitance would be used as signal for biofilm formation and the new type sensor could be made on the base of the observation. We presume that the peak could be attributed to spin resonance of organic polymer derived from BF.
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Academic Significance and Societal Importance of the Research Achievements |
バイオフィルムは細菌が各種材料表面に付着することにより形成される不均一な膜状物質である.これが形成されることにより,衛生状況の劣化,材料の腐食劣化への移行,スケールの形成,慢性病の誘発など,各種産業分野において様々な問題を引き起こすことが知られている.そのため一方においてはこれを抑制する必要性があり,また他方においては,その進行状況(形成-成長過程)をセンシング,モニタリングする必要性がある.本研究の結果バイオフィルムのモニタリングが可能になる可能性があり,それによって多くの産業上,科学上,工業上の問題が解決される可能性があり,社会派の波及効果は極めて高い.
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Report
(4 results)
Research Products
(125 results)
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[Journal Article] In-situ detection based on the biofilm hydrophilicity for environmental biofilm formation2019
Author(s)
Tanaka, Nobuyuki Kogo, Takeshi Hirai, Nobumitsu Ogawa, Akiko Kanematsu, Hideyuki Takahara, Junko Awazu, Akane Fujita, Nobuko Haruzono, Yoshihide Ichida, Shunji Tanaka, Yo
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Journal Title
Scientific Reports
Volume: 9
Issue: 1
Pages: 8070-8081
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Graphene Dispersed Silane Compound Used as a Coating to Sense Immunity from Biofilm Formation2019
Author(s)
Hideyuki Kanematsu, Ryoichi Nakagawa, Katsuhiko Sano, Dana M. Barry, Akiko Ogawa, Nobumitsu Hirai, Takeshi Kogo, Daisuke Kuroda, Noriyuki Wada, Seung-Hyo Lee, Yoshimitsu Mizunoe
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Journal Title
Medical Devices and Sensors
Volume: 1
Issue: 3-4
Pages: 1-16
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Biofilms By E.coli & S.Epidermidis and Its Sensing Possibility By Graphene-Dispersed Silane Coating2019
Author(s)
Hideyuki Kanematsu, Ryoichi Nakagawa, Katsuhiko Sano, Dana M. Barry, Reo Itoh, Hirohisa Yamada, Nobumitsu Hirai, Hidekazu Miura, Akiko Ogawa, Takeshi Kogo, Daisuke Koruda, Katsuhiko Tsunashima, Katsumi Katakura
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Journal Title
ECS abstract for the 235th annual convention
Volume: 2
Pages: 3-4
Related Report
Open Access / Int'l Joint Research
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[Journal Article] Biofilm formation behaviors formed by E.Coli under weak alternating electromagnetic fields2019
Author(s)
Hideyuki Kanematsu, Takaya Katsuragawa, Dana M. Barry, Keiya Yokoi, Senshin Umeki, Hidekazu Miura, Koji Suzuki, Akiko Ogawa, Nobumitsu Hirai, Takeshi Kogo, Daisuke Kuroda, Stefan Zimmerman
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Journal Title
Ceramic Transaction
Volume: 266
Pages: 195-210
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Evaluation for immunity of Biomaterials Based on Raman spectroscopy2018
Author(s)
Hideyuki Kanematsu, Yuta Sakagami, Dana M. Barry, Michiko Yoshitake, Akiko Ogawa, Nobumitsu Hirai, takeshi Kogo, Daisuke Kuroda, Yoshimitsu Mizunoe
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Journal Title
Proceedings of Materials Science and Technology 2018 (MS&T18), Columbus, Ohio, the USA
Volume: 1
Pages: 1482-1489
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nanocomposite polymer film for anti biofouling materials surfaces2018
Author(s)
Hideyuki Kanematsu, Katsuhiko Sano, Hajime Ikegami, Dana M. Barry, Michiko Yoshitake, Yoshimitsu Mizune, Toshihiro Tanaka
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Journal Title
In Fundamentals of nanoparticles - Classifications, Synthesis Methodds, Properties and Characterization, Barhoum, A. and A. S. H. Makhlouf Eds.; Elsevier: Amsterdam, Netherlands
Volume: 1
Pages: 105-128
DOI
ISBN
9780323512558
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Polymer Brush Made by Ionic Liquids and the Inhibition Effects for Biofilm Formation2018
Author(s)
Hideyuki Kanematsu, Atsuya Oizumi, Takaya Sato, Toshio Kamijo, Saika Honma, Dana M. Barry, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Daisuke Kuroda, and Katsuhiko Tsunashima
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Journal Title
ECS Transactions
Volume: 85
Issue: 13
Pages: 1089-1095
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nanofibers and Biofilm in Materials Science2018
Author(s)
Hideyuki Kanematsu, Dana M. Barry, Hajime Ikegai, Michiko Yoshitake, and Yoshimitsu Mizunoe
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Journal Title
Handbook of Nanofibers - Fundamental aspects, experimental setup, synthesis, properties and physicochemical characterization
Volume: 1
Pages: 1-21
DOI
ISBN
9783319427898
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Nanofibers and Biofilm in Materials Science2018
Author(s)
Hideyuki Kanematsu, Dana M. Barry, Hajime Ikegai, Michiko Yoshitake and Yoshimitsu Mizunoe
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Journal Title
Handbook of Nanofibers - Fundamental aspects, experimental setup, synthesis, properties and physicochemical characterization (vol.1
Volume: 1
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Biofilm Formation Behaviors on Graphene by E. coli and S. epidermidis2017
Author(s)
Hideyuki Kanematsu, M Sato, Kodai Shindo, Dana M. Barry, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Yuichi Utsumi, Akinobu Yamaguchi, Hajime Ikegai, Yoshimitsu Mizunoe
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Journal Title
ECS Transaction
Volume: 80
Issue: 10
Pages: 1167-1175
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] クリスタルバイオレットを用いた染色によるバイオフィルムの評価2019
Author(s)
三浦英和, 兼松秀行, 高橋美月, 吉武道子, 平井信充, 幸後健, 小川亜希子, 黒田大介, 和田憲幸, 佐野勝彦, 小林敏郎
Organizer
第29回日本MRS年次大会
Related Report
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[Presentation] 金属材料上に形成されるバイオフィルムの電気化学的モニタリングについて2019
Author(s)
伊藤玲旺, 兼松秀行, 小垣翔, 平井信充, 幸後健, 山田裕久, 片倉勝巳, 綱島克彦, 三浦英和, 小川亜希子, 和田憲幸, 黒田大介
Organizer
第29回日本MRS年次大会
Related Report
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[Presentation] イオン液体から作製されたポリマーブラシとそのバイオフィルム形成/成長挙動について2019
Author(s)
大泉敦也, 兼松秀行, 佐藤涼, 佐藤貴哉, 上條利夫, 本間彩夏, 綱島勝彦, 平井信充, 幸後健, 小川亜希子, 黒田大介
Organizer
第29回日本MRS年次大会
Related Report
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[Presentation] CVDグラフェンとバイオフィルム感受性について2019
Author(s)
兼松秀行, 中川遼一, 斉藤開, 三浦英和, 石原正統, 伴雅人, 平井信充, 幸後健, 小川亜希子, 黒田大介, 和田憲幸, 佐野勝彦, 小林敏郎, Dana M. Barry
Organizer
第29回日本MRS年次大会
Related Report
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[Presentation] Interaction between Graphene Surfaces and Extracellular Polymeric Substances of Biofilms2019
Author(s)
Hideyuki Kanematsu, Ryoichi Nakagawa, Dana M. Barry, Katsuhiko Sano, Masatou Ishihara, Masahito Ban, Noriyuki Wada, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Daisuke Kuroda
Organizer
Materials Science and Technology 2019 (MS&T 2019)
Related Report
Int'l Joint Research / Invited
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[Presentation] イオン液体を用いたポリマーブラシコーティング上への環境常在菌によるバイオフィルム形成挙動とその除去について2019
Author(s)
兼松秀行, 大泉敦也, 佐藤涼, 佐藤貴哉, 上條利夫, 本間彩夏, 綱島勝彦, 平井信充, 幸後健, 小川亜希子, 黒田大介
Organizer
第140回表面技術協会講演大会(福岡工業大学)
Related Report
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[Presentation] Biofilms formed on implanted materials and the analysis of the components: From the viewpoint of correlation between organic polymers and materials.2019
Author(s)
Kanematsu, Hideyuki, Barry, Dana M, Hirai, Nobumits, Ogawa, Akiko, Wada, Noriyuki, Kogo, Takeshi, Kuroda, Daisuke
Organizer
4th International Conference on Innovations in Biomaterials, Biomanufacturing, and Biotechnologies (Bio4)
Related Report
Int'l Joint Research / Invited
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[Presentation] Organic Contamination on Ionic Liquid Polymer Brush and Its Analysis By Raman Spectroscopy2019
Author(s)
Hideyuki Kanematsu, Atsuya Oizumi, T.Sato, T.Kamijo, S.Honma, D. M.Barry, A.Ogawa, N.Hirai, T.Kogo, D.Kuroda, K.Tsunashima, M.Yoshitake
Organizer
the 235th convention of ECS Inc. Sheraton Hotel & Convention Center
Related Report
Int'l Joint Research
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[Presentation] Biofilms By E.coli & S.Epidermidis and Its Sensing Possibility By Graphene-Dispersed Silane Coating2019
Author(s)
Hideyuki Kanematsu, Ryoichi Nakagawa, Katsuhiko Sano, Dana M. Barry, Reo Itoh, Hirohisa Yamada, Nobumitsu Hirai, Hidekazu Miura, Akiko Ogawa, Takeshi Kogo, Daisuke Kuroda, Katsuhiko Tsunashima, Katsumi Katakura
Organizer
The 235th convention of ECS Inc. Sheraton Hotel & Convention Center, Dallas, TX, the USA
Related Report
Int'l Joint Research
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[Presentation] Electrochemical Behaviors of Some Metallic Materials during Biofilm Formations in LB Liquid Culture with Escherichia coli2019
Author(s)
Hideyuki Kanematsu, Akito Tazaki, Reo Itoh, Hirohisa Yamada, Dana M. Barry, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Daisuke Kuroda, Katsuhiko Tsunashima, Katsumi Katakura
Organizer
The 235th Convention of ECS Inc. Hotel Sheraton & Convention Center, Dallas Downtown
Related Report
Int'l Joint Research
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[Book] Fundamentals of Nanoparticles2018
Author(s)
Hideyuki Kanematsu, Katsuhiko Sano, Hajime Ikegami, Dana M. Barry, Michiko Yoshitake, Yoshimitsu Mizune, Toshihiro Tanaka
Total Pages
666
Publisher
Elsevier
ISBN
9780323512558
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