Application of heat shock response for enhanced growth characteristics of microalgae

• Project/Area Number: 17K06924
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: KATSUDA Tomohisa 神戸大学, 工学研究科, 准教授 (50335460)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ヘマトコッカス プルビアリス / クロレラ ソロキニアナ / フローサイトメトリー / 熱ショック応答 / 細胞周期 / 細胞同調 / 化学工学 / バイオテクノロジー / バイオリアクター / 微細藻 / 増殖制御

Outline of Final Research Achievements

In the photoautotrophic cultivation of microalgae, the effects of circadian variation in cultivation temperature and illuminated light intensity were investigated. The temperature variation between the optimal temperature under static condition and that as hot as the cells stop growting resulted in an increased growth rate by 1.4 - 2 times and reducing the lag phase by 1 d in comparison with those under the optimal static condition. These improvements in growth characteristics caused from synchronization of cell cycle and was found in taxonomically different class of green algae.

Academic Significance and Societal Importance of the Research Achievements

CO2 を利用して色素などの有用物質を生産できる微細藻類は，価格競争力のある工業生産のために，増殖性の優れた藻株や効率的な培養法が求められている．本研究によって明らかになった手法は，種々の微細藻類に対して適用可能であるとともに，培養温度や照射光強度を維持するためのエネルギー消費を削減することも期待できるため，微細藻類を利用した有用物質の生産に広く役立つことが期待される．

Research Products

• [Int'l Joint Research] University of Nottingham Malaysia Campus(マレーシア)
• [Journal Article] Integration process for betacyanins extraction from peel and flesh of Hylocereus polyrhizus using liquid biphasic electric flotation system and antioxidant activity evaluation (2019)
  • Author(s): Hui Yi Leong, Chien Wei Ooi, Chung Lim Law, Advina Lizah Julkifle, Tomohisa Katsuda, Pau Loke Show
  • Journal Title: Separation and Purification Technology Volume: 209 Pages: 193-201
  • DOI: 10.1016/j.seppur.2018.07.040
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Green technology of liquid biphasic flotation for enzyme recovery utilizing recycling surfactant and sorbitol (2018)
  • Author(s): Revathy Sankaran, Pau Loke Show, Yee Jiun Yap, Yang Tao, Tau Chuan Ling, Katsuda Tomohisa
  • Journal Title: Clean Technologies and Environmental Policy Volume: 20 Issue: 9 Pages: 2001-2012
  • DOI: 10.1007/s10098-018-1523-5
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Surface grafting techniques on the improvement of membrane bioreactor: State-of-the-art advances (2018)
  • Author(s): Xin Jiat Lee, Pau Loke Show, Tomohisa Katsuda, Wei-Hsin Chen, Jo-Shu Chang
  • Journal Title: Bioresource Technology Volume: 269 Pages: 489-502
  • DOI: 10.1016/j.biortech.2018.08.090
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 微細藻の細胞分裂に及ぼす培養温度・光強度の周期的制御の影響 (2019)
  • Author(s): 勝田 知尚, 森本 高章, 桑井 仁葵, 山地 秀樹
  • Organizer: 第71回日本生物工学会大会
• [Presentation] Effects of the circadian control in temperature and light intensity on the cell division of green algae under a photoautotrophic condition (2019)
  • Author(s): Tomohisa Katsuda, Hiroyuki Yamada, Takaaki Morimoto, Hitoki Kuwai, Hideki Yamaji
  • Organizer: 25th Young Asian Biological Engineering Community
  • Int'l Joint Research
• [Presentation] Photoautotrophic growth of Haematococcus pluvialis under controlled temperature and light conditions (2019)
  • Author(s): Tomohisa Katsuda
  • Organizer: Mahidol-Kobe Workshop
  • Int'l Joint Research / Invited
• [Presentation] 緑藻 Chlorella sorokiniana の光独立栄養培養における培養温度の影響 (2018)
  • Author(s): 桑井 仁葵, 山地 秀樹, 勝田 知尚
  • Organizer: 化学工学会第50回秋季大会
• [Presentation] 培養温度・光強度の周期的な変化が緑藻 Haematococcus pluvialis の細胞分裂に及ぼす影響 (2018)
  • Author(s): 森本 高章, 山地 秀樹, 勝田 知尚
  • Organizer: 化学工学会第50回秋季大会
• [Presentation] 緑藻Haematococcus pluvialisにおける周期的温度変化と細胞増殖の関係 (2018)
  • Author(s): 勝田 知尚，森本 高章，山地 秀樹
  • Organizer: 化学工学会第83年会
• [Presentation] エアリフト式フォトバイオリアクターの操作条件と微細藻類の光独立栄養増殖の関係 (2017)
  • Author(s): 紅林 愛夏，森本 高章，西村 拓海，山地 秀樹，勝田 知尚
  • Organizer: 化学工学会第49回秋季大会
• [Remarks] 神戸大学工学研究科応用化学専攻 生物プロセス工学研究室
  • URL: http://www2.kobe-u.ac.jp/~katsuda/KindexJ.html