| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Outline of Final Research Achievements |
Organisms produce diverse enzymes for metabolism. Enzymes serve as excellent catalysts that exhibit substrate and steric specificities and can be used in various industrial fields. However, enzymes are generally instable even under temperate conditions where organisms survive, and therefore, numerous enzymes cannot be industrially utilized despite having beneficial catalytic activities. In this study, we developed new platforms to generate thermostable (stable) enzyme variants from instable enzymes. The outcomes include not only efficient approaches to generate random mutant libraries that use thermophilic cells as host but also those to screen thermostable enzyme variants in the libraries.
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