• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction of Psychrophile-based simple biocatalysts for the efficient bioconversion with multi-step reactions

Research Project

Project/Area Number 17K06927
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionHiroshima University

Principal Investigator

Takahisa Tajima  広島大学, 統合生命科学研究科(先), 助教 (80571116)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords酵素触媒 / 低温菌 / イタコン酸 / 融合酵素 / cis-アコニット酸デカルボキシラーゼ / アコニターゼ / リンカー / 融合タンパク質 / クエン酸 / 代謝酵素 / 酵素活性 / 熱処理 / 多段階反応
Outline of Final Research Achievements

In this study, we constructed the psychrophile-based simple biocatalysts for the efficient bioconversion with heat treatment. Most of the metabolic enzymes in host psychrophilic bacterium were inactivated with heat treatment at normal temperature. we also constructed the fusion proteins of two conversion enzymes. Some of them showed the tendency of increased efficiency. They also had the advantage in the leak of conversion enzymes from the immobilized biocatalyst.

Academic Significance and Societal Importance of the Research Achievements

本研究は環境負荷の少ない生物学的な物質変換法としてシンプル酵素触媒の効率化に着目して行った。本触媒は簡便な操作で変換反応に不要な副産物生成経路を削除できることから有用化学品の収率向上に大きく貢献すること、融合酵素による反応及び固定化触媒での酵素漏出に寄与することが示された。以上の成果はバイオ触媒の効率性の向上と今後のさらなる触媒改変に重要な知見であると考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (30 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (25 results) (of which Int'l Joint Research: 10 results,  Invited: 2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] インドネシア甘味料・繊維作物研究所(Balittas)(インドネシア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Accelerating itaconic acid production by increasing membrane permeability of whole-cell biocatalyst based on a psychrophilic bacterium Shewanella livingstonensis Ac102020

    • Author(s)
      G. Luo, M. Fujino, S. Nakano, A. Hida, T. Tajima, J. Kato
    • Journal Title

      Journal of Biotechnology

      Volume: 312 Pages: 56-62

    • DOI

      10.1016/j.jbiotec.2020.03.003

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] シンプル酵素触媒によるイタコン酸の生産2019

    • Author(s)
      田島誉久
    • Organizer
      第74回広島大学バイオマスイブニングセミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 低温菌シンプル酵素触媒によるイタコン酸の効率的生産2019

    • Author(s)
      田島誉久,羅宮臨風,中野翔,緋田安希子,加藤純一
    • Organizer
      環境バイオテクノロジー学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Psychrophile-based Simple Biocatalyst for Efficient Conversion2019

    • Author(s)
      Takahisa Tajima, Luo Gonglinfeng, Sho Nakano, Akiko Hida, Junichi Kato
    • Organizer
      The 14th Asian Congress on Biotechnology (ACB2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Carboxylic acid production by psychrophlie-based simple catalyst2019

    • Author(s)
      Gonglinfeng Luo, Syo Nakano, Takahisa Tajima, Junichi Kato
    • Organizer
      The 3rd International Symposium on Fuels and Energy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シンプル酵素触媒における酵素の高機能化関する研究2019

    • Author(s)
      藤野美穂、羅宮臨風、中野翔、田島誉久、緋田安希子、加藤純一
    • Organizer
      生物工学会若手研究者の集い夏のセミナー2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低温菌Shewanella livingstonesis Ac10における熱透過全細胞触媒の開発2019

    • Author(s)
      羅 宮臨風,田島誉久
    • Organizer
      第77回広島大学バイオマスイブニングセミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Itaconic acid production by a marine psychrophilic bacterium2019

    • Author(s)
      Takahisa Tajima, Luo Gonglinfeng, Junichi Kato
    • Organizer
      Marine Biotechnology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Efficient production of itaconic acid using a psychrophile-based simple biocatalyst2019

    • Author(s)
      Gonglinfeng Luo, Takahisa Tajima, Junichi Kato
    • Organizer
      第71回日本生物工学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低温菌シンプル酵素触媒を活用したイタコン酸の効率的な生産2019

    • Author(s)
      中野翔,羅宮臨風,田島誉久,緋田安希子,加藤純一
    • Organizer
      日本農芸化学会2019年度西日本・中四国支部合同沖縄大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イタコン酸変換酵素の高機能化に関する研究2019

    • Author(s)
      藤野美穂,羅宮臨風,中野翔,田島誉久,緋田安希子,加藤純一
    • Organizer
      日本農芸化学会2019年度西日本・中四国支部合同沖縄大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低温菌を活用したシンプル酵素触媒による効率的な物質変換2019

    • Author(s)
      田島誉久
    • Organizer
      広島大学若手研究者による研究シーズ発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Efficient bioproduction by psychrophile-based simple biocatalyst2019

    • Author(s)
      Takahisa Tajima, Gonglinfeng Luo, Syo Nakano, Miho Fujino and Junichi Kato
    • Organizer
      25th Symposium of Young Asian Biological Engineers’ Community
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Simultaneous production of 3-Hydroxypropionic acid and 1,3-Propanediol by psychrophile-based simple biocatalysts in Shewanella sp.2019

    • Author(s)
      Mohammad Mojarrad, Takahisa Tajima, Akiko Hida, Junichi Kato
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 低温菌を活用したシンプル酵素触媒構築のための基盤技術の開発2018

    • Author(s)
      鴨木 明子, 田島 誉久, 小西 正朗, 八久保 晶弘, 坂上 寛敏, 南 尚嗣, 山下 聡, 中島田 豊, 加藤 純一
    • Organizer
      日本農芸化学会中四国支部第51回講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] Simultaneous production of 3-Hydroxypropionic acid and 1,3-Propanediol by psychrophile-based simple biocatalysts in Shewanella sp.2018

    • Author(s)
      Mohammad MOJARRAD, Takahisa Tajima, Junichi Kato
    • Organizer
      The 2nd International Symposium on Fuels and Energy
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Carboxylic acid production by psychrophlie-based simple catalyst2018

    • Author(s)
      Luo GONGLINFENG, Takahisa Tajima, Junichi Kato
    • Organizer
      The 2nd International Symposium on Fuels and Energy
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 低温菌シンプル酵素触媒の宿主とその培養に関する基盤的解析2018

    • Author(s)
      鴨木明子, 田島誉久, 小西正朗, 八久保晶弘, 坂上寛敏, 南尚嗣, 山下聡, 中島田豊, 加藤純一
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 低温菌を活用したシンプル酵素触媒によるジカルボン酸の効率的生産2018

    • Author(s)
      中野翔, 羅宮臨風, 田島誉久, 加藤純一
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 北海道東沖海底堆積物より取得した低温性微生物の解析2018

    • Author(s)
      田島 誉久, 鴨木 明子, 小西 正朗, 八久保 晶弘, 坂上 寛敏, 南 尚嗣, 山下 聡, 中島田 豊, 加藤 純一
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Carboxylic acid production by psychrophlie-based simple catalyst2017

    • Author(s)
      Luo Gonglinfeng, Takahisa Tajima, Yutaka Nakashimada, Junichi Kato
    • Organizer
      The 1st International Symposium on Fuels and Energy
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Bioproduction by psychrophile-based simple biocatalysts2017

    • Author(s)
      Takahisa Tajima, Mai Hamada, Koji Fuki, Yutaka Nakashimada, Junichi Kato
    • Organizer
      The 1st International Symposium on Fuels and Energy
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Efficient conversion system with NADH regeneration by the psychrophile-based simple biocatalyst2017

    • Author(s)
      Kazumasa Mori, Ami Tsuzuki, Takahisa Tajima, Yutaka Nakashimada, Junichi Kato
    • Organizer
      The 1st International Symposium on Fuels and Energy
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 低温菌シンプル酵素触媒における補酵素再生系に関する研究2017

    • Author(s)
      森一将、津々木亜美、田島誉久、廣田隆一、黒田章夫、中島田豊、加藤純一
    • Organizer
      生物工学若手研究者の集い2017夏のセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] 低温菌シンプル酵素触媒における補酵素再生系に関する研究2017

    • Author(s)
      森一将, 津々木亜美, 田島誉久, 廣田隆一, 黒田章夫, 中島田豊, 加藤純一
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Efficient conversion by psychrophile-based simple biocatalysts2017

    • Author(s)
      Takahisa Tajima, Mai Hamada, Koji Fuki, Yutaka Nakashimada, Junichi Kato
    • Organizer
      The 23rd Symposium of Young Asian Biological Engineers' Community (YABEC 2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 低温菌を用いたシンプル酵素触媒の開発

    • URL

      https://home.hiroshima-u.ac.jp/~mbiotech/kato_lab/psychrophile.html

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
  • [Remarks] 低温菌を活用したシンプル酵素触媒による効率的な物質変換

    • URL

      https://hutdb.hiroshima-u.ac.jp/seeds/view/1077

    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 低温菌を用いたイタコン酸の製造方法2018

    • Inventor(s)
      田島誉久、加藤純一、羅宮臨風
    • Industrial Property Rights Holder
      田島誉久、加藤純一、羅宮臨風
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-124796
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi