| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we attempted to establish an efficient culture process to obtain endodermal progenitor cells from iPS cells using an original three-dimensional culture method using hollow fibers.
We achieved a high-cell-density culture of iPS cells in the hollow fibers by culturing cells under the undifferentiated condition and switching to differentiation-inducing condition. The differentiation efficacy of iPS cells in hollow fiber culture was higher than that of monolayer culture. We also found that the size of hollow fibers affected the differentiation fate of iPS cells. Furthermore, we attempted to induce endodermal differentiation under high-cell-density conditions using a suitable size of the hollow fiber. We found that approximately 45% of endodermal progenitor cells were obtained in the differentiation culture.
These results indicated that the hollow fiber culture would contribute to establishing an efficient differentiation culture process for iPS cells.
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