• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Escherichia coli for fuel cells that highly integrates useful functions for biofuel cells

Research Project

Project/Area Number 17K06932
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionOsaka City University

Principal Investigator

Azuma Masayuki  大阪市立大学, 大学院工学研究科, 教授 (20285282)

Co-Investigator(Kenkyū-buntansha) 尾島 由紘  大阪市立大学, 大学院工学研究科, 准教授 (20546957)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsバイオ燃料電池 / 微生物燃料電池 / 微生物触媒 / 大腸菌 / 代謝 / MFC
Outline of Final Research Achievements

In recent years, with the improvement of the performance of biofuel cells, its practical application is strongly expected. In this research, improvement of the microbial catalyst, which is the key point of its development, was promoted. Specifically, using Escherichia coli, which is a model organism, as a catalyst, useful functions were highly integrated using a chromosome modification technique. We focused on mutations in genes involved in the central metabolic pathway of glucose used as fuel. From among these, we found a mutation that leads to an improvement in performance, constructed a strain with 5 gene defects (Δ5) by stacking them, and succeeded in improving the power and coulombic efficiency. We also found a new candidate mutation by evaluating the relationship between performance and the defects of genes related to the functions of the inner and outer membranes. In the future, improvement is expected from the elucidation of the part of Δ5 that limits power generation.

Academic Significance and Societal Importance of the Research Achievements

微生物燃料電池(MFC)に関する研究は、廃水や廃棄物からの発電と廃棄物の減容や、木質系バイオマスを燃料にした地産地消の再生可能エネルギー開発などにも繋がり、社会的にも重要な課題でもある。また、MFCは有機物から細胞内の代謝を介して電子を取りだす装置であり、微生物触媒に関する研究は「細胞内での電子生成および電子伝達メカニズムの理解」「電子の生成や流れを人為的に制御する手法の確立」などに関わり、生命現象を電子の流れで理解し応用展開する新たな「電子生命工学分野」の発展にも貢献する。また、電子の供給から物質生産性を高める電気培養分野にも有益な情報を提供するなど、学術的にも意義深い研究である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Promoted performance of microbial fuel cells using Escherichia coli cells with multiple-knockout of central metabolism genes2019

    • Author(s)
      Ojima Yoshihiro、Kawaguchi Taichi、Fukui Saki、Kikuchi Ryota、Terao Kazuma、Koma Daisuke、Ohmoto Takashi、Azuma Masayuki
    • Journal Title

      Bioprocess and Biosystems Engineering

      Volume: 43 Issue: 2 Pages: 323-332

    • DOI

      10.1007/s00449-019-02229-z

    • NAID

      120006900905

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019679

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Construction of superior catalyst suitable for microbial fuel cell and effect of anode composition on power of the fuel cell2019

    • Author(s)
      Ryota Kikuchi, Taichi Kawaguchi, Daisuke Koma, Takashi Omoto, Yoshihiro Ojima,Masayuki Azuma
    • Organizer
      The 14th Asian Congress on Biotechnology (ACB2019), Tamsui, Taiwan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 種々の遺伝子改変の組み合わせが微生物燃料電池の出力に与える影響2018

    • Author(s)
      川口太一, 菊池亮太, 駒大輔, 大本貴士,  尾島由紘, 東雅之
    • Organizer
      日本生物工学会大会平成30年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 木質系バイオマスを燃料にした微生物発電2018

    • Author(s)
      東雅之, 五十嵐幸一, 尾島由紘
    • Organizer
      イノベーション・ジャパン2018, 東京ビッグサイト(大学組織展示)
    • Related Report
      2018 Research-status Report
  • [Presentation] 大腸菌の代謝や細胞表層電子伝達系の改変が微生物燃料電池の性能に与える影響2017

    • Author(s)
      川口 太一, 福井 早紀, 駒 大輔, 大本 貴士, 尾島 由紘, 東 雅之
    • Organizer
      日本生物工学会大会平成29年度大会
    • Related Report
      2017 Research-status Report
  • [Book] Current Topics in Biochemical Engineering, Catalyst Development of Microbial Fuel Cells for Renewable-Energy Production2018

    • Author(s)
      Azuma Masayuki、Ojima Yoshihiro
    • Total Pages
      19
    • Publisher
      IntechOpen
    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi