| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We have previously reported that PCR amplification efficiency decreased when the template DNA containing VEGF G-quadruplex (G4) was CpG methylated. In this project, thermal stability of the CpG methylated VEGF G4 structure was analyzed. The results revealed that the G4 structure was stabilized by CpG methylation in the presence of 20 mM NaCl and 2 mM MgCl2. Thermal analysis also demonstrated that c-kit1 G4 structure was destabilized by N6-methyladenosine modifications. Moreover, 9,651 G4 clusters were identified in human genomic DNA by high-throughput sequencing of whole genome amplified products with a G4 ligand.
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