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Analysis on the mechanisms of circuit formation of multi-layered inter-areal connections in the cerebral cortex

Research Project

Project/Area Number 17K07076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionOsaka University

Principal Investigator

Oka Yuichiro  大阪大学, 連合小児発達学研究科, 講師 (30614432)

Co-Investigator(Kenkyū-buntansha) 佐藤 真  大阪大学, 連合小児発達学研究科, 教授 (10222019)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords神経回路網 / 神経回路形成 / 大脳皮質 / 長連合線維 / plexin / 皮質内回路 / 組織透明化 / イメージング / sparse labeling / Kv channel / サブプレート / 神経科学
Outline of Final Research Achievements

Direct connections between functional areas in the cerebral cortex is essential for higher cortical functions. However, developmental processes and mechanisms of such connections are yet to be fully elucidated. We analyzed axon projections of Plxnd1-expressing neurons in layers 2/3 and 5a in mouse cerebral cortex and found that ipsilateral inter-areal projections are formed as one of the axon collaterals budding from earlier-forming callosal axons connecting bilateral cerebral hemispheres. The collateral for inter-areal projection extended more rapidly than those for local circuit within an area. We also identified the Kcnab1 gene as expressed in layer 6b neurons with inter-areal projections and revealed its expression changes during cortical development and axon projections.

Academic Significance and Societal Importance of the Research Achievements

本研究は、独自に構築した大脳皮質領野間回路を単一ニューロンレベルで選択的に可視化する系を用いて、領野間の回路の形成過程を明らかにした。領野間回路は軸索側枝によるものであることから、その形成機構の解明は、複数領域への投射による脳内の並列回路の形成機構の解明に資するものである。自閉スペクトラム症にみられる運動機能の低下が1次体性感覚野と1次運動野の直接的な神経連絡の乱れと関連することが知られており、連合性回路の形成機構の解明により、その病態理解につながる可能性がある。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (26 results)

All 2020 2019 2018 2017 Other

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (19 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA receptor density and PSD95 turnover2019

    • Author(s)
      Xie Min-Jue、Ishikawa Yasuyuki、Yagi Hideshi、Iguchi Tokuichi、Oka Yuichiro、Kuroda Kazuki、Iwata Keiko、Kiyonari Hiroshi、Matsuda Shinji、Matsuzaki Hideo、Yuzaki Michisuke、Fukazawa Yugo、Sato Makoto
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 4305-4305

    • DOI

      10.1038/s41598-019-40838-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kcnab1 is expressed in subplate neurons with unilateral long-range inter-areal projections2019

    • Author(s)
      Tiong Y.X.S., Oka Y., Sasaki T., Taniguchi M., Doi M., Akiyama H., Sato, M.
    • Journal Title

      Frontiers in Neuroanatomy

      Volume: 印刷中 Pages: 39-39

    • DOI

      10.3389/fnana.2019.00039

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Overlapping but distinct topology for zebrafish V2R-like olfactory receptors reminiscent of odorant receptor spatial expression zones.2018

    • Author(s)
      Ahuja G., Reichel V., Kowatschew D., Syed A.S., Kotagiri A.K., Oka Y., Weth F., Korsching SI.
    • Journal Title

      BMC Genomics

      Volume: 19 Issue: 1 Pages: 383-383

    • DOI

      10.1186/s12864-018-4740-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] An early subplate marker Kcnab1 is expressed in cortico-cortical projection neurons in layer 6b in the mouse neocortex.2020

    • Author(s)
      Oka Yuichiro, Tiong Sheena Y.X., Yahaya Murtala Hamza, Sasaki, Tatsuya, Doi Miyuki, Yasumura Misato, Taniguchi Manabu, Sato Makoto.
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 発達段階の大脳新皮質において、Hsd11b1発現領域は一時的に拡大する2020

    • Author(s)
      土井美幸、岡雄一郎、佐藤真
    • Organizer
      第125回日本解剖学会総会・全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Kcnab1 is an early marker for subplate of the mouse cerebral cortex and expressed in cortico-cortical projection neurons in layer 6b2019

    • Author(s)
      Yuichiro Oka, Sheena Y. X. Tiong, Tatsuya Sasaki, Miyuki Doi, Manabu Taniguchi, Makoto Sato
    • Organizer
      Neuro2019 (第42回日本神経学会大会/第62回日本神経化学大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単一大脳皮質2/3層ニューロンによる長連合性および交連性回路の形成過程の解析2019

    • Author(s)
      岡 雄一郎、土井 美幸、谷口 学、猪口 徳一、佐藤 真
    • Organizer
      第46回日本脳科学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Long associational projections of Plxnd1-expressing cortical neurons are formed by interstitial collaterals from the callosally-projecting primary axonal shaft2018

    • Author(s)
      Oka Y., Lin Y., Tiong Y.X.S., Sasaki T., Doi M., Iguchi T., Sato M.
    • Organizer
      22nd Biennial Meeting of the International Society for Developmental Neuroscienc
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Elevated extension speed for a selected long associational collateral of a single cortical dual-projection neuron2018

    • Author(s)
      Oka Y., Lin Y., Tiong Y.X.S., Sasaki T., Doi M., Iguchi T., Sato M.
    • Organizer
      2018 Joint Meeting between The Neurodevelopmental Biology Section of The Korean Society for Molecular and Cellular Biology and Japanese Developmental Neuroscientist
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Potassium channel subunit Kcnab1 is expressed in cortical layer 6b long-range corticocortical projection neurons2018

    • Author(s)
      Tiong S., Oka Y., Sasaki T., Sato M.
    • Organizer
      11th FENS Forum of Neuroscience
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 単一皮質ニューロンは軸索毎に異なる伸長速度により複数領野へ同調的に投射する2018

    • Author(s)
      岡雄一郎, 林由佳, Sheena Y. X. Tiong, 佐々木達也, 土井美幸, 猪口徳一, 佐藤真
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report
  • [Presentation] バレル皮質におけるPlxnd1発現第5a層ニューロンの軸索伸展様式の解析2018

    • Author(s)
      林由佳, 岡雄一郎, 佐藤真
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Analysis on axonal development of Plxnd1-expressing L5a neurons in the barrel cortex2018

    • Author(s)
      Y. Lin, Y. Oka, M. Sato
    • Organizer
      第11回神経発生討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] Inter-areal collateral branches of layer 2/3 neurons grow more rapidly than intra-areal collateral branches in the mouse neocortex2018

    • Author(s)
      Makoto Sato, Yuichiro Oka
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 脳発生における軸索側枝形成を制御する受容体群の役割について2018

    • Author(s)
      猪口 徳一、Nguyen Quynh Mai、安村 美里、吉田 知之、岡 雄一郎、佐藤 真
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 大脳皮質ニューロン軸索側枝による領野間回路形成過程の解析2018

    • Author(s)
      岡 雄一郎、林 由佳、Tiong Sheena、土井 美幸、佐々木 達也、猪口 徳一、佐藤 真
    • Organizer
      第95回日本生理学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Molecular characterisation and axon projection pattern of deep cortical layer 6 neurons in mouse2017

    • Author(s)
      張 永欣, 岡 雄一郎, 佐藤 真
    • Organizer
      第64回日本生化学会近畿支部例会
    • Related Report
      2017 Research-status Report
  • [Presentation] Developmental analysis of the inter-areal connections from cortical neurons in different layers2017

    • Author(s)
      Y. Oka, Y. Lin, M. Hattori, S.Y.X. Tiong, M. Doi, T. Iguchi, M. Sato
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Antero-posterior organization of pontine nuclei contributes to the connection of cortico-ponto-cerebellar pathway through the expression of axon guidance molecules2017

    • Author(s)
      T. Iguchi, M. Omi, Y. Oka, M. Sato
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] A potassium channel subunit as a novel molecular marker for long-range projection neurons in the earliest-formed cortical layer2017

    • Author(s)
      S.Y.X. Tiong, Y. Oka, T. Sasaki, M. Sato
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Distant area-targeting axonal branches of layer 2/3 neurons develop more rapidly than locally targeting branches in mouse neocortex2017

    • Author(s)
      Y. Oka, Y. Lin, M. Hattori, S.Y.X. Tiong, M. Doi, T. Sasaki, T. Iguchi, M. Sato
    • Organizer
      第48回生理研国際シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 発生過程における一次体性感覚野内の第5層神経細胞の軸索側枝伸展2017

    • Author(s)
      林由佳, 岡雄一郎, 佐藤真
    • Organizer
      第93回日本解剖学会近畿支部学術集会
    • Related Report
      2017 Research-status Report
  • [Remarks] 大阪大学大学院医学系研究科 解剖学講座(神経機能形態学)

    • URL

      http://www.anat2.med.osaka-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 大阪大学大学院医学系研究科解剖学講座(神経機能形態学)

    • URL

      http://www.anat2.med.osaka-u.ac.jp/index.html

    • Related Report
      2018 Research-status Report
  • [Remarks] 解剖学講座(神経機能形態学)

    • URL

      http://www.anat2.med.osaka-u.ac.jp/index.html

    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 非相同末端結合と相同組換えを組み合わせたゲノム編集ノックイン法2019

    • Inventor(s)
      真下知士 , 岡雄一郎 (6番目)ら (計7名)
    • Industrial Property Rights Holder
      真下知士 , 岡雄一郎 (6番目)ら (計7名)
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-072782
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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