• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis on the centrosomal functions of neural dendrites - microtubule nucleation and minus-end anchoring

Research Project

Project/Area Number 17K07083
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionSophia University

Principal Investigator

HAYASHI Kensuke  上智大学, 理工学部, 教授 (50218567)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords脳の発達 / 神経細胞 / 樹状突起 / 微小管 / 中心体 / 物質輸送 / ニューロン / 細胞・組織 / 脳・神経 / 発生・分化 / 神経科学
Outline of Final Research Achievements

Microtubules must increase in number for dendrite growth. The centrosome of a neuron lacks the ability to form microtubules, and it has been unclear where microtubules are produced in neurons. In this study, we showed that microtubules are nucleated in the cytoplasm in neurons. Furthermore, we found a neuron-specific alternative splicing isoform of CDK5RAP2, as a protein that activates microtubule formation in the cytoplasm of neurons. In cells expressing this isoform, microtubule formation was induced in the cytoplasm. These results indicate that selective splicing of CDK5RAP2 is involved in the increase of microtubules in developing neurons.

Academic Significance and Societal Importance of the Research Achievements

脳の発達に必要な、神経細胞の樹状突起の成長をコントロールする新しい仕組みが明らかになった。この仕組みを応用して、脳の機能発達に介入するあたらしい技術の開発への道を開いた。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (20 results)

All 2022 2021 2020 2019 2018 2017

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (14 results)

  • [Journal Article] The difference in the cytoskeletal machinery of growth cones of growing axons and leading processes2022

    • Author(s)
      Miyata Kaho、Hayashi Kensuke
    • Journal Title

      Developmental Neuroscience

      Volume: 1 Issue: 6 Pages: 1-1

    • DOI

      10.1159/000522200

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Golgi apparatus is the main microtubule-organizing center in differentiating skeletal muscle cells2021

    • Author(s)
      Ide Koyo、Muko Mika、Hayashi Kensuke
    • Journal Title

      Histochemistry and Cell Biology

      Volume: 156 Issue: 3 Pages: 273-281

    • DOI

      10.1007/s00418-021-01999-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of a Potent Anticancer Agent PVHD303 with in Vivo Activity2020

    • Author(s)
      Suzuki Yumiko、Otake Ayana、Ueno Satoshi、Hayashi Kensuke、Ishii Hirosuke、Miyoshi Nao、Kuroiwa Kenta、Tachikawa Masashi、Fujimaki Yuki、Nishiyama Kotaro、Manabe Kei、Yamazaki Ryuta、Asai Akira
    • Journal Title

      ACS Medicinal Chemistry Letters

      Volume: 11 Issue: 6 Pages: 1287-1291

    • DOI

      10.1021/acsmedchemlett.0c00119

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microtubule nucleation in the cytoplasm of developing cortical neurons and its regulation by brain‐derived neurotrophic factor2019

    • Author(s)
      Yamada Mimori、Hayashi Kensuke
    • Journal Title

      Cytoskeleton

      Volume: 76 Issue: 5 Pages: 339-345

    • DOI

      10.1002/cm.21550

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] KATNAL1 is a more active and stable isoform of katanin, and is expressed dominantly in neurons2018

    • Author(s)
      Hatakeyama Eiko、Hayashi Kensuke
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 507 Issue: 1-4 Pages: 389-394

    • DOI

      10.1016/j.bbrc.2018.11.048

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Changes in the axo-glial junctions of the optic nerves of cuprizone-treated mice2018

    • Author(s)
      Wataru Kojima and Kensuke Hayashi
    • Journal Title

      Histochemistry and Cell Biology

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Difference between two katanin isoforms in the neuronal expression and ubiquitination2020

    • Author(s)
      Suzuki S. and Hayashi K.
    • Organizer
      日本分子生物学会
    • Related Report
      2020 Research-status Report
  • [Presentation] Interaction between cytosolic dynein and neuronal cytosolic isoform of microtubule anchoring protein, ninein.2020

    • Author(s)
      Nakayama M. and Hayashi K.
    • Organizer
      日本分子生物学会
    • Related Report
      2020 Research-status Report
  • [Presentation] ニューロンの細胞質からの微小管形成と微小管形成タンパクCDK5RAP2のエキソンスキップ2019

    • Author(s)
      中村 朱里   林 謙介
    • Organizer
      分子生物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 軸索突起の伸長と先導突起の移動におけるフィロポディアおよびラメリポディアの役割2019

    • Author(s)
      宮田夏帆 林謙介
    • Organizer
      分子生物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Changes of microtubule arrangement induced by neuron-specific ninein isoform2018

    • Author(s)
      Moeka Hara and Kensuke Hayashi
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 筋細胞の分化過程におけるゴルジ体からの微小管形成2018

    • Author(s)
      井出晃陽、林謙介
    • Organizer
      日本動物学会関東支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニューロンの細胞質で微小管を形成するgamma-TuRC2018

    • Author(s)
      山田美森、林謙介
    • Organizer
      日本動物学会関東支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 神経の発生における微小管切断酵素katanin A-like1の役割2018

    • Author(s)
      畠山瑛子、林謙介
    • Organizer
      日本動物学会関東支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニューロン特異的nineinアイソフォームの発現と機能2018

    • Author(s)
      原 萌夏、林 謙介
    • Organizer
      日本動物学会関東支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 神経細胞の微小管形成とCDK5RAP2スプライシングバリアント2018

    • Author(s)
      中村朱里、林 謙介
    • Organizer
      日本動物学会関東支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 移動性ニューロンの先導突起形成におけるdrebrinの働き2018

    • Author(s)
      宮田夏帆、林 謙介
    • Organizer
      日本動物学会関東支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Changes in the microtubule organizing center during differentiation of muscle cells2017

    • Author(s)
      Koyo Ide, Kensuke Hayashi
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Microtubule nucleation in the cytoplasm of developing neurons2017

    • Author(s)
      M. Yamada , K. Hayashi
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Characterization of microtubule severing enzyme, katanin A-like1, highly expressed in neurons2017

    • Author(s)
      E. Hatakeyama and K. Hayashi
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi