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Exploring the mechanism of the entry of endoplasmic reticulum into dendritic spines for modifying excitatory synaptic transmission

Research Project

Project/Area Number 17K07108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNagoya University

Principal Investigator

Ageta-Ishihara Natsumi  名古屋大学, 理学研究科, 講師 (60547561)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords細胞骨格 / 学習 / 記憶
Outline of Final Research Achievements

Discrimination among different spatial contexts depends on the hippocampal dentate gyrus (DG). However, molecular basis remains unclear. Here we find that Septin-null (Sept-KO) mice retain memory of spatial context for 2h, but not for 24h, in spatial pattern separation test. To narrow down brain regions responsible for the cognitive impairment, we conduct local subacute depletion of SEPT from the bilateral DG of wild-type mice, and local supplementation of SEPT into the bilateral DG of KO mice, which respectively recapitulates and rescues the defect. The perforant path-DG synapses in KO mice are normal, except for a significant scarcity of smooth endoplasmic reticulum (sER), a Ca2+ reservoir in dendritic spines. The sER anomaly is recapitulated by SEPT depletion from primary cultured rat DG granule cells (DGCs). The DGC-selective requirement of SEPT will illuminate a novel gating or translocation mechanism that regulates the entry of sER from dendritic shaft into spines.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、学術的には細胞小器官の移動が記憶保持に影響を及ぼすことを示す初めての概念となる。社会的には、認知症患者と診断される数年前の時点でも空間pattern separation機能が低下することが報告されていることから、空間弁別課題の長期記憶保持の基盤となる分子メカニズムの解明は薬剤探索や記憶保持機能低下を抑制する食品の開発に繋がることが期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (16 results)

All 2020 2019 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results)

  • [Journal Article] CDC42EP4, a perisynaptic scaffold protein in Bergmann glia, is required for glutamatergic tripartite synapse configuration.2018

    • Author(s)
      Ageta-Ishihara N, Konno K, Yamazaki M, Abe M, Sakimura K, Watanabe M, Kinoshita M.
    • Journal Title

      Neurochem Int

      Volume: S0197-0186 Issue: 17 Pages: 30442-4

    • DOI

      10.1016/j.neuint.2018.01.003

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] UBL3 modification influences protein sorting to small extracellular vesicles.2018

    • Author(s)
      Ageta H, Ageta-Ishihara N, Hitachi K, Karayel O, Onouchi T, Yamaguchi H, Kahyo T, Hatanaka K, Ikegami K, Yoshioka Y, Nakamura K, Kosaka N, Nakatani M, Uezumi A, Ide T, Tsutsumi Y, Sugimura H, Kinoshita M, Ochiya T, Mann M, Setou M, Tsuchida K.
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 3936-3936

    • DOI

      10.1038/s41467-018-06197-y

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Activity- and septin-dependent extension of smooth ER into dendritic spines as a synaptic basis of memory consolidation2020

    • Author(s)
      小坂優介、上田(石原)奈津実、深澤有吾、見学美根子、高雄敬三、宮川剛、 尾藤晴彦、木下専
    • Organizer
      2019年度文部科学省新学術領域研究 学術研究支援基盤形成 先端モデル動物支援プラットフォーム成果発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A synaptic basis of spatial pattern separation in the hippocampal dentate gyrus is activity- and septin-dependent extension of smooth endoplasmic reticulum into dendritic spines2020

    • Author(s)
      Ageta-Ishihara Natsumi, Asami Yurika, Fukumasu Naoto, Sakakibara Kodai, Fuse Naoki, Mitsui Riku, Fukazawa Yugo, Kengaku Mineko, Takao Keizo, Miyakawa Tsuyoshi, Inokuchi Kaoru, Bito Haruhiko, Kinoshita Makoto
    • Organizer
      第16回成体脳のニューロン新生懇談会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A novel UBL3 modification influences protein sorting to small extracellular vesicles2019

    • Author(s)
      Ageta Hiroshi, Ageta-Ishihara Natsumi, Hitachi K, Onouchi T, Yamaguchi H, Yoshioka Y, Kosaka N, Ide T, Kinoshita M, Ochiya T, Setou M, Tsuchida K.
    • Organizer
      ISEV2019, Symposium session: EV Biogenesis II
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 神経活動とセプチンに依存した樹状突起棘への小胞体侵入による記憶の長期化2019

    • Author(s)
      木下専, 上田(石原)奈津実, 深澤有吾, 宮川剛, 高雄啓三, 尾藤晴彦
    • Organizer
      第42回日本神経科学大会 第62回日本神経化学大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 空間弁別の個性を形成する環境的要因と遺伝的要因の複合解析2019

    • Author(s)
      上田(石原)奈津実、木下専
    • Organizer
      「個性」創発脳第四回領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 個性の階層的理解を目指した評価系の確立 -空間弁別の分子・細胞学的基盤 -2019

    • Author(s)
      上田(石原)奈津実、榊原広大、福枡直人、藤原里沙子、小坂優介、高雄啓三、宮川剛、尾藤晴彦、井ノ口馨、深澤有吾、木下専
    • Organizer
      「個性」創発脳第四回領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 環境要因により影響を受ける認知機能に対するアプローチ2019

    • Author(s)
      上田(石原)奈津実、木下専
    • Organizer
      資生堂女性研究者サイエンスグラント受賞者 特別講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 長期記憶保持に必要な分子・細胞基盤の解明2019

    • Author(s)
      上田(石原)奈津実、木下専
    • Organizer
      2019 GTR Seeds Seminar
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] An activity-regulated septin subunit SEPT3 is required for the entry of sER into dendritic spines and spatial discrimination.2018

    • Author(s)
      Ageta-Ishihara N, Fukazawa Y, Takao K, Miyakawa T, Bito H, Inokuchi K,
    • Organizer
      Gordon Research Conference “Cell Biology of the Neuron”
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Spatial discrimination defects caused by chronic, systemic loss of a septin subunit is recapitulated by its acute, local depletion in the hippocampal dentate gyrus2018

    • Author(s)
      Ageta-Ishihara N, Takao K, Miyakawa T, Hioki H, Bito H, Inokuchi K, Kinoshita M.
    • Organizer
      第1回国際シンポジウム “Towards Understanding INDIVIDUALITY”
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Requirement of a septin subunit SEPT3 in the dentate gyrus granule cells for dendritic spine maturation and spatial discrimination2018

    • Author(s)
      Sakakibara K, Fukumasu N, Ageta-Ishihara N, Fukazawa Y, Takao K, Miyakawa T, Bito H, Kinoshita M.
    • Organizer
      The 6th Neural Circuits Joint Workshop
    • Related Report
      2018 Research-status Report
  • [Presentation] Unexpected motor and histopathological phenotype found in mice that lack SEPT7 in the cerebellum2018

    • Author(s)
      Ageta-Ishihara N, Kurita H, Yamazaki M, Abe M, Fukazawa Y, Sakimura K, Kinoshita M.
    • Organizer
      The 6th Neural Circuits Joint Workshop
    • Related Report
      2018 Research-status Report
  • [Presentation] A septin-dependent synaptic regulation required for spatial discrimination2018

    • Author(s)
      上田(石原)奈津実, 木下 専
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 空間弁別に必要なセプチン依存的シナプス制御2017

    • Author(s)
      上田(石原)奈津実, 深澤有吾, 鈴木悠大, 増田啓吾, 高雄啓三, 宮川剛, 尾藤晴彦, 木下 専
    • Organizer
      第40回神経科学学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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