| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Axons have a specific cytoskeletal structure lining the cytoplasmic face of the axolemma. Super-resolution microscopy has revealed a remarkable periodic lattice in axons. αII-spectrin is the only α spectrin detected in the nervous system, and forms the periodic lattice in axons. While αII-spectrin intracellular localization is fairly well understood, the molecular mechanism by which αII-spectrin was regulated remains unclear. In this study, we report that Cdk5 phosphorylates αII-spectrin. We identified the phosphorylation site of αII-spectrin by Cdk5, and made anti-phospho-specific αII-spectrin antibody. The phosphorylated αII-spectrin was localized in the axon initial segment. Inhibition of Cdk5 impaired axon initial segment formation and neuronal polarity. These results suggest that Cdk5 regulates the axonal periodic cytoskeleton through the phosphorylation of αII-spectrin.
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