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The elucidation of molecular mechanism of Epithelial ovarian cancer (EOC) model mouse

Research Project

Project/Area Number 17K07129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionTohoku University

Principal Investigator

MIYOSHI ICHIRO  東北大学, 医学系研究科, 教授 (10183972)

Co-Investigator(Kenkyū-buntansha) 北村 浩  酪農学園大学, 獣医学群, 教授 (80312403)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords実験動物学 / 卵巣癌モデルマウス / 遺伝子組換えマウス / ゲノム編集 / がん化の分子機構 / 卵管特異的糖タンパク質(OGP)
Outline of Final Research Achievements

pithelial ovarian cancer (EOC) is a gynecological disease that has a high mortality rate and difficult to detect early stage and treat efficiently.. There is no appropriate mouse models, since the origin of EOC have not been identified. Recently, many reports suggested that most EOCs may not originate from the ovarian surface epithelium, but from the female genital tract. The EOC mouse model generated by Miyoshi et al. exhibits a progression from female genital tract, in spite of the tumor of the other most mouse models develop from the ovarian surface epithelium. In the present study, we generated a knock-in mouse carrying the simian virus 40 (SV40) large T-antigen (Tag) gene and the enhanced green fluorescent protein (EGFP) gene in the site of mouse oviduct-specific glycoprotein (OGP) gene to better understand the biology of the tumor and develop new approaches for EOC prevention, detection, and treatment.

Academic Significance and Societal Importance of the Research Achievements

上皮性卵巣癌は最も死亡率の高い婦人科悪性腫瘍であるが,その発生母地が不明なこと,病態の解析に相応しい動物モデルがないことが診断・治療法の開発の障害であった。近年,上皮性卵巣癌の発生母地が卵巣上皮から卵管上皮(内癌)へパラダイムシフトしつつあり,申請者が樹立したトランスジェニックモデルマウスが有用なモデルとして評価されている。しかし,同マウスは,子宮・膣も腫瘍化してしまう問題があった。本研究では,卵管上皮細胞特異的に発癌・可視化する新規遺伝子組換えマウスを作製し,卵巣癌のがん化初期の分子機構を明らかにすると共に,診断・治療の標的分子やマーカーの探索研究に応用することが可能となる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (17 results)

All 2019 2018 2017 Other

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (6 results) (of which Invited: 6 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Ganglioside Synthase Knockout Reduces Prion Disease Incubation Time in Mouse Models2019

    • Author(s)
      Kobayashi Atsushi、Qi Zechen、Shimazaki Taishi、Munesue Yoshiko、Miyamoto Tomomi、Isoda Norikazu、Sawa Hirofumi、Aoshima Keisuke、Kimura Takashi、Mohri Shirou、Kitamoto Tetsuyuki、Yamashita Tadashi、Miyoshi Ichiro
    • Journal Title

      The American Journal of Pathology

      Volume: 189 Issue: 3 Pages: 677-686

    • DOI

      10.1016/j.ajpath.2018.11.009

    • NAID

      120006770947

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Contribution of rat embryonic stem cells to xenogeneic chimeras in blastocyst or 8-cell embryo injection and aggregation.2019

    • Author(s)
      Okumura H, Nakanishi A, Toyama S, Yamanoue M, Yamada K, Ukai A, Hashita T, Iwao T, Miyamoto T, Tagawa YI, Hirabayashi M, Miyoshi I, Matsunaga T.
    • Journal Title

      Xenotransplantation

      Volume: 26(1) Issue: 1

    • DOI

      10.1111/xen.12468

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A domain responsible for spontaneous conversion of bank vole prion protein2018

    • Author(s)
      Kobayashi Atsushi、Matsuura Yuichi、Takeuchi Atsuko、Yamada Masahito、Miyoshi Ichiro、Mohri Shirou、Kitamoto Tetsuyuki
    • Journal Title

      Brain Pathology

      Volume: 29 Issue: 2 Pages: 155-163

    • DOI

      10.1111/bpa.12638

    • NAID

      120006803949

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 個別基準の解説(第4章個別基準) 実験等を行う施設の運用に関する解説 (特集 教育セミナーフォーラム2018 実験動物の飼養及び保管並びに苦痛の軽減に関する基準解説書 : 改訂の背景並びに基準の適正な理解と運用のために) -- (基準の適正運用と解説書の活用)2018

    • Author(s)
      三好一郎
    • Journal Title

      LABIO 21 (1345-9147)

      Volume: 73 Pages: 12-15

    • NAID

      40021637819

    • Related Report
      2018 Research-status Report
  • [Journal Article] Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells2017

    • Author(s)
      Kitamura Hiroshi、Ishino Takeshi、Shimamoto Yoshinori、Okabe Jun、Miyamoto Tomomi、Takahashi Eiki、Miyoshi Ichiro
    • Journal Title

      Mediators of Inflammation

      Volume: 2017 Pages: 1-15

    • DOI

      10.1155/2017/6909415

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Macrophage ubiquitin-specific protease 2 modifies insulin sensitivity in obese mice.2017

    • Author(s)
      Saito N, Kimura S, Miyamoto T, Fukushima S, Amagasa M, Shimamoto Y, Nishioka C, Okamoto S, Toda C, Washio K, Asano A, Miyoshi I, Takahashi E, Kitamura H.
    • Journal Title

      Biochem Biophys Rep.

      Volume: 9 Pages: 322-329

    • DOI

      10.1016/j.bbrep.2017.01.009

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Animal ethics and welfare education in wet-lab training can foster residents’ ethical values toward life2017

    • Author(s)
      Iki Y, Ito T, Kudo K, Noda M, Kanehira M, Sueta T, Miyoshi I, Kagaya Y, Okada Y, Unno M
    • Journal Title

      Experimental Animals

      Volume: 66 Issue: 4 Pages: 313-320

    • DOI

      10.1538/expanim.17-0026

    • NAID

      130006187189

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 大学における学生・研究者を対象とした教育訓練2019

    • Author(s)
      三好一郎
    • Organizer
      日動協教育フォーラム2019
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 個別基準:その1 実験等を行う施設2018

    • Author(s)
      三好一郎
    • Organizer
      第65回日本実験動物学会総会 動物福祉・倫理委員会シンポジウム(招待講演)
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 実験動物の飼養及び保管並びに苦痛の軽減に関する基準の解説―個別基準:実験等を行う施設―2018

    • Author(s)
      三好一郎
    • Organizer
      日本実験動物技術者協会北海道支部第43回支部通常総会講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] ブタを用いた動物実験への看護的視点の導入2017

    • Author(s)
      末田輝子,三好一郎,笠井憲雪
    • Organizer
      第160回日本獣医学会学術集会,日本実験動物医学会シンポジウム
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 外部検証,認証による動物実験機関管理の促進2017

    • Author(s)
      三好一郎
    • Organizer
      第64回日本実験動物学会総会 動物福祉・倫理委員会シンポジウム
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 飼育技術者が行う看護的飼育ケアの実際ー技術者が説く動物実験計画書ー2017

    • Author(s)
      末田輝子,三好一郎,笠井憲雪
    • Organizer
      第42回日本実験動物技術者協会関東支部懇話会シンポジウム
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] 発生工学.獣医学教育モデル・コア・カリキュラム準拠実験動物学(第2版,久和茂編)2018

    • Author(s)
      三好一郎
    • Total Pages
      228
    • Publisher
      朝倉書店
    • ISBN
      9784254460360
    • Related Report
      2017 Research-status Report
  • [Book] 4章個別基準4-1実験を行う施設.実験動物の飼養及び保管並びに苦痛の軽減に関する基準の解説(環境省自然環境局総務課動物愛護管理室編)2017

    • Author(s)
      三好一郎(実験動物飼養保管基準解説書研究会)
    • Total Pages
      198
    • Publisher
      アドスリー
    • ISBN
      9784904419724
    • Related Report
      2017 Research-status Report
  • [Book] 実験動物・動物実験施設の取組み 大学等.明日の幸せは健康から(改定版)NPO法人動物実験関係者連絡協議会編2017

    • Author(s)
      三好一郎
    • Total Pages
      36
    • Publisher
      アドスリー
    • Related Report
      2017 Research-status Report
  • [Remarks] 病態モデル動物の開発を通じて各種疾患のメカニズムを解明し、予防・診断・治療法創出に貢献する

    • URL

      http://www.med.tohoku.ac.jp/about/laboratory/099.html

    • Related Report
      2018 Research-status Report 2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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