Development of an integrated epigenome regulation system and animal model for epigenomic disease

• Project/Area Number: 17K07130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: University of Tsukuba
• Principal Investigator: Iseki Hiroyoshi 筑波大学, 国際統合睡眠医科学研究機構, 助教 (50433652)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エピゲノム / CRISPR / モデル動物

Outline of Final Research Achievements

In this study, we attempted to develop an integrated epigenome regulation system and animal model for epigenomic disease. We developed a novel CRISPR-based system for enhancing gene expression. We further constructed an integrated epigenome regulation system with two CRISPR-associated proteins.

Academic Significance and Societal Importance of the Research Achievements

本研究では、CRISPRを基盤とした統合的エピゲノム制御システムを構築した。エピゲノムを自在に制御できるシステムを構築することは、エピゲノム疾患モデルの作製のみならず、エピゲノムそのものを解析するツールとしても有用であり、また、エピゲノム疾患の治療への応用やiPS細胞誘導等の細胞運命変換への応用なども可能なため、学術的にも社会的にも重要な成果となり得る。

Research Products

• [Journal Article] Cables2 Is a Novel Smad2-Regulatory Factor Essential for Early Embryonic Development in Mice (2019)
  • Author(s): Dinh TTH, Iseki H, Mizuno S, Mizuno S, Tanimoto Y, Daitoku Y, Kato K, Hamada Y, Hasan ASH, Suzuki H, Murata K, Muratani M, Ema M, Kim JD, Ishida J, Fukamizu A, Kato M, Takahashi S, Yagami KI, Wilson V, Arkell R, Sugiyama F.
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/744128
  • Open Access / Int'l Joint Research
• [Presentation] Cables2 regulates mouse gastrulation by stimulating Wnt/β-catenin signalling pathway. (2019)
  • Author(s): Dinh TTH, Iseki H, Mizuno S, Iijima-Mizuno S, Tanimoto Y, Daitoku Y, Kato K, Takahashi S, Yagami K, Sugiyama F.
  • Organizer: 第66回日本実験動物学会総会
• [Presentation] Essential role of Cables2 gene for gastrulation of the mouse embryo (2018)
  • Author(s): Tra Thi Huong Dinh, Hiroyoshi Iseki, Seiya Mizuno, Saori Mizuno, Yoko Tanimoto, Yoko Daitoku, Kanako Kato, Noriko Kajiwara, Masatsugu Ema, Jun-Dal Kim, Junji Ishida, Akiyoshi Fukamizu, Mitsuyasu Kato, Satoru Takahashi, Ken-ichi Yagami, Fumihiro Sugiyama
  • Organizer: 第65回日本実験動物学会総会