Improvement of reproductive technology in rabbit
| Project/Area Number |
17K07138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Saga University |
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Principal Investigator |
Kitajima Shuji 佐賀大学, 総合分析実験センター, 准教授 (70284643)
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| Co-Investigator(Kenkyū-buntansha) |
松久 葉一 佐賀大学, 総合分析実験センター, 助教 (20754253)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ウサギ / 生殖工学 / 系統保存 / 実験動物学 |
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| Outline of Final Research Achievements |
In this study, we established the method for preparing the rabbit embryos effectively as a technique in reproductive engineering in the rabbit. We tried superovulation on the young rabbits (12-14 weeks of age) and found that they have an ability to provide more than 3 times eggs (an average of around 100) comparison with the adult rabbits (20 weeks of age or older) after superovulating with PMSG. After in vitro fertilization (IVF) using eggs collected from young rabbits, there were no significant differences in result of IVF such as fertility and development rate to blastocyst comparison with eggs from adult rabbits. These results indicate that the egg from the young rabbit at least has fertility and the ability of development to blastocyst after IVF.
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| Academic Significance and Societal Importance of the Research Achievements |
医学研究において疾患モデル動物は有用な研究資源であり、これらの保存・供給事業を進めるには、精子・胚の凍結といった保存技術の確立が非常に重要である。マウス・ラットではすでにこれらの技術が確立され、多くの研究機関で広く利用されている。いっぽう、ウサギでは、マウス・ラットに比べ性成熟に時間がかかる上、飼育スペースの確保が難しい、高コストとなるなど系統維持・保存作業は困難な点が多いことから研究が進んでいなかった。本研究の成果は、これらの問題の一端を解決し、ヒト疾患モデルとしての遺伝子改変ウサギ開発の促進や疾患モデルウサギの保存・供給事業の推進に寄与する事ができると考えられれる。
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Macrophage-derived matrix metalloproteinase-1 enhances aortic aneurysm formation in transgenic rabbits.2019
Author(s)
Niimi, M., Nishijima, K., Kitajima, S., Matsuhisa, F., Satoh, K., Yamazaki, H., Zhang, J., Chen, Y.E., Fan, J
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Journal Title
J. Biomed. Res.
Volume: 33
Pages: 271-279
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Growing oocyte specific transcription-dependent de novo DNA methylation at the imprinted Zrsr1-DMR2018
Author(s)
Joh, K., Matsuhisa, F., Kitajima, S., Nishioka, K., Higashimoto, K., Yatsuki, H., Kono, T., Koseki, H., Soejima, H.
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Journal Title
Epigenetics & Chromatin
Volume: 11
Issue: 1
Pages: 28-28
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Increased hepatic expression of endothelial lipase inhibits cholesterol diet induced hypercholesterolemia and atherosclerosis in transgenic rabbits2017
Author(s)
Wang, C., Nishijima, K., Kitajima, S., Niimi, M., Yan, H., Chen, Y., Ning, B., Matsuhisa, F., Liu, E., Zhang, J., Chen, Y. E., Fan. J.
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Journal Title
Arterioscler. Thromb. Vasc. Biol.
Volume: 37
Issue: 7
Pages: 1282-1289
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Effect of inhibin antiserum administration and vulva color on superovulation in rabbit2017
Author(s)
Kazutoshi Nishijima, Gen Watanabe, Shuji Kitajima, Keita Basaki, Yasuyoshi Fukuda, Yukie Komatsu, Megumi Yano, Takahiro Obata, Yuhei Matsuo, Yukihisa Matsuda, Shinsuke Seki
Organizer
The 7th International Meeting on Rabbit Biotechnology
Related Report
Int'l Joint Research
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[Presentation] Increased hepatic expression of endothelial lipase inhibits cholesterol diet induced hypercholesterolemia and atherosclerosis in transgenic rabbits2017
Author(s)
Chuan Wang, Kazutoshi Nishijima, Shuji Kitajima, Manabu Niimi, Hizhao Yan, Yajie Chen, Bo Ning, Fumikazu Matsuhisa, Enqi Liu, Jifeng Zhang, Y. Eugene Chen, Jianglin Fan.
Organizer
The 7th International Meeting on Rabbit Biotechnology
Related Report
Int'l Joint Research
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