| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We defined an extracellular metabolic cue that directs cancer cell migration. First, we devised a novel microfluidic device (GCG) that establishes gradients of pH and O2 concentration in the extracellular medium in standard culture dish. We demonstrated that MDA-MB-231 cells under GCG migrate toward higher pH/O2 regions, whereas directional migration was abolished when extracellular pH gradients were eliminated by hepes buffer. Extracellular pH in the bulk medium significantly affected directional migration. In contrast, extracellular O2 concentration gradients did not appear to guide the cells. Finally, we have proposed a model for directional cell migration under macroscopic spatial gradients in extracellular pH.
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