Roles of CD239 on adhesion and migration of tumor cells
Project/Area Number |
17K07180
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | Tokyo University of Pharmacy and Life Science |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 細胞接着 / 基底膜 / ラミニン / インテグリン / 幹細胞 / ジストログリカン / CD239 / 癌 / 細胞・組織 / 蛋白質 |
Outline of Final Research Achievements |
CD239 was initially identified as an antigen upregulated on the surface of malignant tumor cells. It is a transmembrane receptor belonging to immunoglobulin superfamily and binds specifically to laminin-511 (trimer of alpha5, beta1, and gamma1 chains) that is a major molecule in the basement membrane of normal and diseased tissues. Therefore, it has been suggested that the interaction of CD239 with laminin-511 is involved in the tumor invasion into the basement membrane. To date, it has been shown that CD239 promotes tumor cell migration on laminin-511, but the mechanism is not fully understood. In this study, we investigated the mechanism of tumor cell migration from the behavior of CD239 at cell surface and demonstrated that CD239 is served as a target antigen for breast tumor.
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Academic Significance and Societal Importance of the Research Achievements |
長い間、ラミニン-511をはじめとする基底膜分子の機能解明は、基底膜の組織からの抽出が難しいため困難であった。これまでに研究代表者は、ラミニン-511を培養細胞の上清から精製できるだけでなく、組換え蛋白質として調製できることを示してきた。本研究は、成体の基底膜に分布するラミニン-511を用いることにより、生体内における癌細胞と基底膜の相互作用をより反映させることができる特色がある。さらに、この精製されたラミニン-511は特異的な受容体であるCD239の機能解明を可能にした。本研究の成果は、基底膜に組込まれた細胞の挙動をコントロールする情報およびその読取りのメカニズムを明らかにすることができる。
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Biological activity of peptide-conjugated polyion complex matrices consisting of alginate and chitosan.2017
Author(s)
Fujimori, C‡., Kumai, J‡., Nakamura, K., Gu, Y., Hozumi, K., Katagiri, F., Kikkawa, Y., and Nomizu, M
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Journal Title
Biopolymers
Volume: 108
Issue: 1
Pages: 356-366
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Human LAMA5 mutation associated with focal segmental glomerulosclerosis2019
Author(s)
Kikkawa Y, Kaimori JY, Namba T, Okazaki A, Kobayashi K, Tanigawa A, Kotani Y, Uno Y, Tomoji M, Ichimaru N, Sekiguchi K, Nakaya A, Takahara S, Nomizu M, Isaka Y
Organizer
ASMB/Vanderbilt 2019 Workshop on Basement Membranes
Related Report
Int'l Joint Research
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[Presentation] Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment2019
Author(s)
Kikkawa Y, Sugawara Y, Hamada K, Yamada, Y, Kumai J, Kanagawa M, Kobayashi K, Toda T, Negishi Y, Katagiri F, Hozumi K, Nomizu M
Organizer
American Society of Cell Biology/Europian Molecular Biology Organization 2019 meeting
Related Report
Int'l Joint Research
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[Presentation] Internalization of CD239 highly expressed in breast cancer cells: a potential antigen for antibody-drug conjugates2018
Author(s)
Kikkawa Y, Enomoto-Okawa Y, Fujiyama A, Fukuhara T, Harashima N, Sugawara Y, Negishi Y, Katagiri F, Hozumi K, Nomizu M, Ito Y
Organizer
American Society of Matrix Biology 2018 Biennial Meeting
Related Report
Int'l Joint Research
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[Presentation] Internalization of CD239, a laminin receptor, in human breast cancer: a novel antigen for antibody-drug conjugates2017
Author(s)
Kikkawa Y, Enomoto-Okawa Y, Fujiyama A, Fukuhara T, Harashima N, Sugawara Y, Ikari K, Negishi Y, Ktagiri F, Hozumi K, Nomizu K, Ito Y
Organizer
2017 Annual Meeting the American Society for Cell Biology
Related Report
Int'l Joint Research
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