Focal copy number alteration and its functional role in carcinogenesis: unmet needs in cancer genome analysis
Project/Area Number |
17K07195
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor diagnostics
|
Research Institution | The University of Tokyo |
Principal Investigator |
Kenji Tatsuno 東京大学, 先端科学技術研究センター, 特任研究員 (80775239)
|
Project Period (FY) |
2017-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | がんゲノム解析 / CNV / HBV / AAV2 / ロングリード解析 / ゲノム構造異常 / MSI / がんゲノム / ゲノム解析 / 癌 |
Outline of Final Research Achievements |
We have developed a new analysis method of the next-generation sequencer (NGS) which can efficiently detect the focal copy number alterations (CNA) and viral genome integration in the cancer genome, which were difficult to detect with conventional NGS analysis. With using this analysis, we have detected a large number of focal copy number alterations in mesotheliomas and virus integration in liver cancers. Furthermore, analysis using a long-read sequencer for mesothelioma and liver cancer revealed that genomic structural abnormalities occurred at the CNA regions and the integration site of the viral genome, which could be cancer driver alterations as well as known single nucleotide variations.
|
Academic Significance and Societal Importance of the Research Achievements |
患者に最適な治療を行うプレシジョンメディシンが開始されているが、現時点では診断される遺伝子変異の種類は限られている。本研究は、これまで解析されてこなかった微小コピー数変化やウイルス挿入によるゲノム構造異常などの探索を、遺伝子パネル検査で使われる次世代シーケンサーの解析技術を応用することで可能とするものであり、がんに存在する多様な変異を逃すことなく解析することで、より精密ながん医療を実現を目指す。また、これまで未解明な部分が多かった微小なゲノム構造異常も、がん化に関連していることが明らかになり、本研究の手法を用いた解析がより詳細な発がんメカニズムの解明につながることが期待される。
|
Report
(5 results)
Research Products
(33 results)
-
-
-
[Journal Article] Base editors for simultaneous introduction of C-to-T and A-to-G mutations2020
Author(s)
Sakata Rina C.、Ishiguro Soh、Mori Hideto、Tanaka Mamoru、Tatsuno Kenji、Ueda Hiroki、Yamamoto Shogo、Seki Motoaki、Masuyama Nanami、Nishida Keiji、Nishimasu Hiroshi、Arakawa Kazuharu、Kondo Akihiko、Nureki Osamu、Tomita Masaru、Aburatani Hiroyuki、Yachie Nozomu
-
Journal Title
Nature Biotechnology
Volume: na
Issue: 7
Pages: 865-869
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Defined lifestyle and germline factors predispose Asian populations to gastric cancer2020
Author(s)
Suzuki A, Katoh H, Komura D, Kakiuchi M, Tagashira A, Yamamoto S, Tatsuno K, Ueda H, Nagae G, Fukuda S, Umeda T, Totoki Y, Abe H, Ushiku T, Matsuura T, Sakai E, Ohshima T, Nomura S, Seto Y, Shibata T, Rino Y, Nakajima A, Fukayama M, Ishikawa S, Aburatani H.
-
Journal Title
Science Advances
Volume: 6
Issue: 19
Pages: 9778-9778
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
[Journal Article] Reduced Neoantigen Expression Revealed by Longitudinal Multiomics as a Possible Immune Evasion Mechanism in Glioma.2019
Author(s)
Nejo T, Matsushita H, Karasaki T, Nomura M, Saito K, Tanaka S, Takayanagi S, Hana T, Takahashi S, Kitagawa Y, Koike T, Kobayashi Y, Nagae G, Yamamoto S, Ueda H, Tatsuno K, Narita Y, Nagane M, Ueki K, Nishikawa R, Aburatani H, Mukasa A, Saito N, Kakimi K.
-
Journal Title
Cancer Immunol Res
Volume: in press
Issue: 7
Pages: 1148-1161
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Epigenetic landscape influences the liver cancer genome architecture2018
Author(s)
Natsuko Hama, Yasushi Totoki, Fumihito Miura, Kenji Tatsuno, Mihoko Saito-Adachi, Hiromi Nakamura, Yasuhito Arai, Fumie Hosoda, Tomoko Urushidate, Shoko Ohashi, Wakako Mukai, Nobuyoshi Hiraoka, Hiroyuki Aburatani , Takashi Ito and Tatsuhiro Shibata
-
Journal Title
Nature Communications
Volume: 9
Issue: 1
Pages: 1643-1643
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Comprehensive and Integrative Genomic Characterization of Hepatocellular Carcinoma2017
Author(s)
Ally Adrian, Balasundaram Miruna,,,,Aburatani Hiroyuki, Yamamoto Shogo, Tatsuno Kenji,,,et al
-
Journal Title
Cell
Volume: 169
Issue: 7
Pages: 1327-1341.e23
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Distinct molecular profile of diffuse cerebellar gliomas2017
Author(s)
Nomura M, Mukasa A, Nagae G, Yamamoto S, Tatsuno K, Ueda H, Fukuda S, Umeda T, Suzuki T, Otani R, Kobayashi K, Maruyama T, Tanaka S, Takayanagi S, Nejo T, Takahashi S, Ichimura K, Nakamura T, Muragaki Y, Narita Y, Nagane M, Ueki K, Nishikawa R, Shibahara J, Aburatani H, Saito N
-
Journal Title
Acta Neuropathologica
Volume: 134
Issue: 6
Pages: 941-956
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Presentation] All-in-one panel assay for clinical cancer sequencing2018
Author(s)
Kenji Tatsuno, Shinji Kosaka, Hiroki Ueda, Shogo Yamamoto, Shuhei Yoshikawa, Toshihide Ueno, Takashi Aoki, Shiro Fukuda, Kunihiro Nishimura, Hiroyuki Mano, Hiroyuki Aburatani
Organizer
Precision Medicine in Cancer, Keystone Symposia
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-