| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
I investigated the development of evolutionary molecular engineering selection method of cell penetrating peptides (CPP) for RNAi intracellular delivery based on gene suppression efficiency. I modified the mRNA for cDNA display selection of CPP with shRNA sequence at 5’ terminus in order to select the CPP based on both of cellular uptake efficiency and gene suppression efficiency. In this study, I developed the method of preparation of shRNA-conjugated cDNA display complex and confirmed the gene expression efficiency of shRNA domain after cell transfection. However, it was also revealed that the yield of shRNA-conjugated cDNA display complex should be improved for the CPP selection using cultured cells based on gene suppression level.
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