| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We found that early histone genes are transcriptionally active during morula stage and that multiple early histone gene loci interact each other during this stage. Furthermore, transcriptional active state of these gene loci is involved in this chromosomal interactions. For live imaging analysis of these loci, we attempted to establish the CRISPR-Cas9 system and achieved 100% knockout efficiency in sea urchin embryos using CRISPR-Cas9. Currently, we constructed a imaging tool based on this Cas9 that is available for sea urchin and the tuning of this tool is still ongoing. In addition, we analyzed the intranuclear structure of sea urchin embryos by using fluorescent proteins and found that the centromere showed a biased distribution in the nucleus like Rabl configuration and that multiple spots of the nucleolus tended to be close together.
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