| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The MIBP1 (HIVEP2) gene encodes a 270 kDa protein that acts as a transcription factor, by binding to specific DNA sequences. Loss of function mutations in the MIBP1 gene have been associated with intellectual disabilities, suggesting that MIBP1 is crucial for proper brain development and function.
To identify target genes for transcriptional regulation by MIBP1 protein, we tried to insert FLAG-tag sequence to endogenous MIBP1 gene by genome editing of various cell lines. One of established cell lines, M15, which is derived from HCT116 colon cancer cells have both wild type and edited allele. MIBP1 expression was induced immediately after TNF-alpha treatment from the edited allele as well as the wild type allele. Increased mRNA expression was accompanied by enhanced expression of FLAG-tagged MIBP1 protein. As a result of genome editing, changes in mRNA stability were observed.
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