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the mechanism that eliminates transcription noise and maintains cell uniformity

Research Project

Project/Area Number 17K07247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genome biology
Research InstitutionNagoya University

Principal Investigator

Suzuki Miho  名古屋大学, 医学系研究科, 助教 (80548470)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords転写 / lncRNA / スプライシング / pre-mRNA / エピゲノム / DNAメチル化 / 細胞均一性
Outline of Final Research Achievements

To elucidate the mechanism that regulates the uniformity and variation of gene expression levels among individual cells, we focused on RNA molecules retained in the nucleus and analyzed them. We found that RNAs that require more time for pre-mRNA splicing are more likely to stay in the nucleus. In addition, we found that lncRNAs stocked in the nucleus, specifically in cancer cells, are involved in the regulation of DNA replication. This study shows that nuclear RNAs play an important role in the maintenance of cellular homeostasis.

Academic Significance and Societal Importance of the Research Achievements

これまで、mRNAが遺伝子から転写された後、すみやかに細胞質へ運ばれて翻訳されることはわかっていたが、あえて核内にとどまるRNAの存在やその生物学的意義についてはよくわかっていなかった。本研究により、限られたスペースに多くのタンパク質や核酸が詰め込まれた細胞核の中で、DNA複製などの細胞に必須の現象がRNAによって時間空間的に制御されていることがわかった。このようなRNAはしばしば疾患特異的に過剰発現しており、治療のターゲットとして期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-Wild-Type Diffuse Astrocytic Gliomas2019

    • Author(s)
      Ohka Fumiharu、Shinjo Keiko、Deguchi Shoichi、Matsui Yusuke et al.
    • Journal Title

      Cancer Research

      Volume: 79 Issue: 19 Pages: 4814-4827

    • DOI

      10.1158/0008-5472.can-19-1272

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] S期進行における lncRNA TUG1 の機能2019

    • Author(s)
      鈴木美穂, 飯島健太, 勝島啓佑, 室伏善照, 新城恵子, 近藤豊
    • Organizer
      第13回日本エピジェネティクス研究会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Regulation of Genome Integrity by Direct and Immediate Interaction between lncRNA-1 and Replication Protein A2019

    • Author(s)
      Suzuki M, Iijima K, Katsushima K, Murofushi Y, Shinjo K, Kondo Y
    • Organizer
      Abcam Epigenetics Conference and 14th Asia Epigenome Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA複製阻害により発現上昇するlncRNA(長鎖非翻訳RNA)の機能2019

    • Author(s)
      鈴木美穂, 飯島健太, 室伏善照, 新城恵子, 近藤豊
    • Organizer
      日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The biological function of invertebrate DNA methylation in nucleosomal positioning2018

    • Author(s)
      Miho Suzuki
    • Organizer
      Chromatin Structure and Function Gordon Research Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 転写伸張領域のDNAメチル化による転写共役スプライシング効率の調節2017

    • Author(s)
      鈴木美穂
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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