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Adaptation to multiple stress environments by nucleoid compaction

Research Project

Project/Area Number 17K07279
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Molecular biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

Ryosuke Ohniwa  筑波大学, 医学医療系, 准教授 (30447883)

Co-Investigator(Kenkyū-buntansha) 森川 一也  筑波大学, 医学医療系, 教授 (90361328)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords核様体 / 全遺伝子発現解析 / 黄色ブドウ球菌 / 細菌遺伝学 / 環境ストレス / 酸化ストレス / MrgA / 凝集 / 遺伝子発現プロファイル / 環境応答 / 遺伝子発現制御 / 細菌
Outline of Final Research Achievements

The aim of this study is to clarify the adaptation mechanism of bacteria against multiple stress environments by nucleoid compaction. Through the analysis of Staphylococcal nucleoid condensation by MrgA, we showed that 1)MrgA binding to the nucleoid without oxidative stresses up-regulated the gene expression of non-coding genes distributed genome-widely, and 2) MrgA binding to the nucleoid with oxidative stresses up-regulated and/or down-regulated the gene expression of coding-genes distributed only around ori-region (replication initiation region).

Academic Significance and Societal Importance of the Research Achievements

従来、複合的なストレス環境に対する適応システムは、個別の環境因子を感知して応答する独立した遺伝子発現系の総体という考え方であった。本研究により、核様体凝集という現象を介した複数のストレス環境への適応、という従来と異なる環境適応進化戦略を提案した。
また、染色体(核様体)全体の構造変化が、ゲノム位置依存的な遺伝子発現を制御するという、新しい見解を提案できた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2017

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 3 results)

  • [Journal Article] What Happens in the Staphylococcal Nucleoid Under Oxidative Stress?2019

    • Author(s)
      Kazuya Morikawa, Yuri Ushijima , Ryosuke L. Ohniwa, Masatoshi Miyakoshi and Kunio Takeyasu
    • Journal Title

      Microorganisms

      Volume: 7 Issue: 12 Pages: 631-648

    • DOI

      10.3390/microorganisms7120631

    • NAID

      120007132875

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generating process of Emerging Topics in the life sciences2019

    • Author(s)
      Ohniwa Ryosuke L, Hibino Aiko
    • Journal Title

      Scientometrics

      Volume: 121 Issue: 3 Pages: 1549-1561

    • DOI

      10.1007/s11192-019-03248-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Finding of Agr phase variants in Staphylococcus aureus.2019

    • Author(s)
      Vishal Gor, Aya J. Takemura, Masami Nishitani, Masato Higashide, Veronica Medrano Romero, Ryosuke L. Ohniwa, and Kazuya Morikawa
    • Journal Title

      mBio

      Volume: 10 Issue: 4

    • DOI

      10.1128/mbio.00796-19

    • NAID

      120007132913

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tks5 and Dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion2019

    • Author(s)
      Chuang Mei-Chun、Lin Shan-Shan、Ohniwa Ryosuke L.、Lee Gang-Hui、Su You-An、Chang Yu-Chen、Tang Ming-Jer、Liu Ya-Wen
    • Journal Title

      Journal of Cell Biology

      Volume: 218 Issue: 5 Pages: 1670-1685

    • DOI

      10.1083/jcb.201809161

    • NAID

      120007133503

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Neutrophil Extracellular Traps Enhance Staphylococcus Aureus Vegetation Formation through Interaction with Platelets in Infective Endocarditis2019

    • Author(s)
      Hsu Chih-Chieh、Hsu Ron-Bin、Ohniwa Ryosuke、Chen Jeng-Wei、Yuan Chang-Tsu、Chia Jean-San、Jung Chiau-Jing
    • Journal Title

      Thrombosis and Haemostasis

      Volume: 119 Issue: 05 Pages: 786-796

    • DOI

      10.1055/s-0039-1678665

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生命へのアプローチ~「型」とデザイン思考~2019

    • Author(s)
      大庭良介
    • Journal Title

      環境会議

      Volume: 2019年春号 Pages: 150-157

    • Related Report
      2018 Research-status Report
  • [Journal Article] Identification of nucleoid associated proteins (NAPs) under oxidative stress in Staphylococcus aureus.2017

    • Author(s)
      Ushijima Y, Ohniwa RL, Morikawa K
    • Journal Title

      BMC Microbiol.

      Volume: 17 Issue: 1 Pages: 207-207

    • DOI

      10.1186/s12866-017-1114-3

    • NAID

      120007134787

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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