Regulation of mRNA metabolism by the transcription-coupled RNA methyltransferase PCIF1
| Project/Area Number |
17K07282
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
Hirose Yutaka 富山大学, 学術研究部薬学・和漢系, 准教授 (00218851)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 遺伝子発現調節 / mRNA生合成 / 転写 / RNA化学修飾 / mRNAキャップ / RNAポリメラーゼII / RNA修飾 / 遺伝子発現 / mRNA / 遺伝子 / 発現制御 |
|---|
| Outline of Final Research Achievements |
I identified PCIF1, a factor that interacts with the phosphorylated C-terminal domain of RNA polymerase II (Pol II), as cap-specific adenosine methyltransferase responsible for N6-methylation of m6Am at the 5’ end of Pol II-transcripts in collaboration with groups in the University of Tokyo and Riken. I also identified novel proteins interacting with PCIF1 in human cells by coimmunoprecipitation and MS analysis.
|
| Academic Significance and Societal Importance of the Research Achievements |
脊椎動物RNAの5’末端に存在するm6Am修飾を触媒する新規酵素を明らかにした本研究は、mRNA化学修飾を介する遺伝子発現制御機構の解明に繋がる。さらにこの化学修飾が、ウイルスや細菌由来のRNAと自己のRNAを識別するためのマークとして機能し、生体防御に重要な役割を果たす可能性が考えられる。従って本研究の成果は、自然免疫応答の新たな機構解明や抗ウイルス薬などの創薬への応用波及効果が期待される。
|
Report
(4 results)
Research Products
(41 results)
-
-
[Journal Article] Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-κB and C/EBPβ on stimulation of Toll-like receptor 9.2017
Author(s)
Yamamoto, S., Hagihara, T., Horiuchi, Y., Okui, A., Wani, S., Yoshida, T., Inoue, T., Tanaka, A., Ito, T., Hirose, Y., and Ohkuma, Y.
-
Journal Title
GENES TO CELLS
Volume: 22
Issue: 3
Pages: 265-276
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
