Emerging links between transcription and RNA export in HIV-1 gene expression
| Project/Area Number |
17K07283
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
Taniguchi Ichiro 京都大学, ウイルス・再生医科学研究所, 助教 (00467432)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HIV-1 / RNA核外輸送 / RNAスプライシング / RNA輸送 / RNA / RNP |
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| Outline of Final Research Achievements |
Gene expression of human immunodeficiency virus type-1 (HIV-1) requires two viral proteins, transcription elongation factor Tat and nuclear RNA export factor Rev, in addition to cellular mechanisms. We found that Tat and Rev bind to a common region of viral RNA. We also faond that Tat enhances but Rev inhibits pre-mRNA splicing in an in vitro reaction system using HIV-1 model RNA. These results suggest that Tat and Rev regulate HIV-1 gene expression by switching the progress of splicing reactions.
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| Academic Significance and Societal Importance of the Research Achievements |
HIV-1タンパク質のTatとRevに焦点を絞った本研究で、TatとRevが連携することによるウイルス遺伝子発現の制御機構を明らかにしつつある。ウイルスタンパク質どうしによる連携はほとんど知られていない。本研究成果は、他のウイルスの増殖機構解明につながる。また、ウイルスタンパク質どうしの連携を標的とした抗ウイルス薬剤開発が期待される。
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Report
(5 results)
Research Products
(14 results)
