| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Gene expression of human immunodeficiency virus type-1 (HIV-1) requires two viral proteins, transcription elongation factor Tat and nuclear RNA export factor Rev, in addition to cellular mechanisms. We found that Tat and Rev bind to a common region of viral RNA. We also faond that Tat enhances but Rev inhibits pre-mRNA splicing in an in vitro reaction system using HIV-1 model RNA. These results suggest that Tat and Rev regulate HIV-1 gene expression by switching the progress of splicing reactions.
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