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Investigation into physical and functional interaction between HUSH complex and RNA

Research Project

Project/Area Number 17K07293
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Molecular biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Hamada Kyoko  基礎生物学研究所, クロマチン制御研究部門, 特別協力研究員 (90450410)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsクロマチン / RNA / 遺伝子発現制御 / ヘテロクロマチン / 転写抑制 / エピジェネティックス
Outline of Final Research Achievements

Recent studies suggest that RNA-binding regulates localization and functions of various chromatin proteins. Here we investigated physical and functional interaction of RNA and MPP8, which is a component of the transcriptional repressor complex called HUSH complex. We found that MPP8 possesses the RNA-binding activity and it interacts with transcripts from a subset of ZNF genes. Furthermore, there was a relatively strong correlation between MPP8-ZNF transcript interaction and nuclear retention of the transcripts. These results indicate that MPP8 or HUSH complex may post-transcriptionally regulate a subset of ZNF genes, implying a novel function of MPP8.

Academic Significance and Societal Importance of the Research Achievements

本研究では、一部の標的遺伝子に対するMPP8もしくはHUSH complexの転写後遺伝子発現制御機能という新規機能を示唆することができた。よって、エピジェネティクス関連分野において新たな知見を生み出すことができた。また、MPP8やHUSH complexはがんや神経変性疾患、感染症との関連が示されていることから、これらの因子の新たな機能が明らかになる事で上記疾患に対する創薬シード探索や創薬研究に貢献できると考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (3 results)

All 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 2 results,  Open Access: 2 results)

  • [Journal Article] Mitotic phosphorylation of HP1α regulates its cell cycle-dependent chromatin binding2019

    • Author(s)
      Gohei Nishibuchi, Shinichi Machida, Reiko Nakagawa, Yuriko Yoshimura, Kyoko Hiragami-Hamada, Yusuke Abe, Hitoshi Kurumizaka, Hideaki Tagami, Jun-ichi Nakayama
    • Journal Title

      The Journal of Biochemistry

      Volume: 165 Issue: 5 Pages: 433-446

    • DOI

      10.1093/jb/mvy117

    • NAID

      40021880612

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Do the charges matter? - Balancing the charges of the chromodomain proteins on the nucleosome.2019

    • Author(s)
      Kyoko Hiragami-Hamada, Jun-ichi Nakayama
    • Journal Title

      The Journal of Biochemistry

      Volume: - Issue: 6 Pages: 455-458

    • DOI

      10.1093/jb/mvz004

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Proteomic analysis of RNA-dependent chromatin association of nuclear proteins2018

    • Author(s)
      Hiragami-Hamada Kyoko、Tani Naoki、Nakayama Jun-ichi
    • Journal Title

      BioRxiv

      Volume: -

    • DOI

      10.1101/391755

    • Related Report
      2018 Research-status Report
    • Open Access / Int'l Joint Research

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

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