Molecular mechanism of autophagosome membrane closure
Project/Area Number |
17K07302
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
Honda Ikuko 東京大学, 大学院医学系研究科(医学部), 特任講師 (10447948)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | オートファジー / オートファゴソーム / ESCRT複合体タンパク質 / membrane fission / リソソーム / 3D-CLEM / 数理モデル / ESCRTタンパク質 / 膜分裂 / 細胞 / 光学顕微鏡 / 生体膜 |
Outline of Final Research Achievements |
In the formation of autophagosome, which is responsible for macroautophagy, it is known that a flat-extended single membrane vesicle closes into a spherical shape and undergoes membrane fission. In this study, we demonstrated that the ESCRT protein complex is required for membrane fission in autophagosome formation. We have also established a method to accurately evaluate the opening and closing of the autophagosome using the 3-Dimensional Correlative Light and Electron Microscopy (3D-CLEM). Furthermore, the process of membrane deformation during autophagosome formation was further understood with the support of a mathematical model.
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、オートファジー研究において遅れていたオートファゴソーム形成後期の分子機構解析を進展させた。また、物理モデルと実験データの融合研究を生み出した。さらに、本研究にて試みた3D-CLEM法のオルガネラ解析への適用は、生命科学分野で広く応用が可能となるだろう。
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Report
(4 results)
Research Products
(18 results)