| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
In the formation of autophagosome, which is responsible for macroautophagy, it is known that a flat-extended single membrane vesicle closes into a spherical shape and undergoes membrane fission. In this study, we demonstrated that the ESCRT protein complex is required for membrane fission in autophagosome formation. We have also established a method to accurately evaluate the opening and closing of the autophagosome using the 3-Dimensional Correlative Light and Electron Microscopy (3D-CLEM). Furthermore, the process of membrane deformation during autophagosome formation was further understood with the support of a mathematical model.
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