| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Polysaccharides are composed of various monosaccharide residues linked with glycosidic bonds. The 3D structures of polysaccharide are supposed to exist as dynamic equilibrium of multiple conformations. Compared with nucleic acids and proteins, the investigation of the relationship between sequence and 3D structure of polysaccharide has been not fully understood yet. This is because of high flexibility of 3D structure of polysaccharide and technical difficulties of structural analyses. In this proposal, I proposed the structural analysis of N-glycan which is representative glycan in mammals by X-ray crystallography. I and co-workers clarified several atomic structures of N-glycan and its related glycoproteins in this three years. Especially, we reported the crystal structure of cancer-associated glycosyltransferase GnT-V in complex with ligand N-glycan. This report has the potential to clarify the relationship between cancer specific glycan and its behavior.
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