| Project/Area Number |
17K07305
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
Tama Florence 名古屋大学, 理学研究科, 教授 (20648191)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 2D images / XFEL / Cryo-EM / Biomolecules / structure / dynamics / 3D structure / single molecules / molecular shapes / database / single-particle |
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| Outline of Final Research Achievements |
In this project, we have been developing new efficient approaches to find 3D biological shapes from a few EM or XFEL images to serve as a starting point for further data analyses. In this approach, databases of known molecular shapes are assembled and numerical algorithms are used to identify the shapes that are consistent with a few query images.
We had first developed the protocol for using EM real space images as inputs to test the feasibility of the approach. We showed that a small number of images can be sufficient as query images to identify similar 3D shapes. Then we have been developing algorithms to use XFEL diffraction patterns as inputs. Since simulations of XFEL diffraction patterns is time-consuming, we have developed a new approach to use Gaussian mixture model to model the structure. We have also the algorithms to automatically identify the region with strong information in the diffraction patterns to identify matches.
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| Academic Significance and Societal Importance of the Research Achievements |
生体分子の構造に関する情報はそれらの機能を理解して医薬などの応用に活用するために重要である。本研究ではデータベースを活用することによりクライオ電子顕微鏡やX線自由電子レーザーによる観測データからターゲットの形を素早く推定するための手法を開発した。
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