| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Musashi1 (Msi1), which is involved in proliferative maintenance of cancer and stem cells, has two RNA-binding domains, RBD1 and RBD2. We firstly elucidated the driving force of how RBD1 recognizes the target RNA in highly specific manner by means of statistical mechanics of hydration. We also determined the NMR structure of the RBD2:target RNA complex, by which we elucidated the interaction mode. We then built a structural model of the Msi1 RBD1-RBD2:target RNA complex, and demonstrated that Msi1 can bind to the target RNAs having different lengths. As for FUS, a causal agent for neurodegenerative diseases, we demonstrated that it exhibits large conformational change upon target RNA binding.
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