| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To clarify structural changes in nucleosomes caused by modifications, such as DNA methylation and histone acetylation, we performed biophysical analysis of modified nucleosome core particles (NCPs) that were reconstituted in vitro. In the analysis of modified NCPs, mono- and di-NCP were prepared with 147 and 366 base-pair (bp) DNA oligomers. Further, we prepared NCPs with DNA oligomers having AT or CG rich sequences, and characterized their biophysical properties by mass spectrometry and small angle X-ray scattering. No structural information is available for these NCPs due to difficulty in crystallization. We established a mass spectrometric method to reliably determine the methylation levels of DNA fragments with ~400 bp.
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