| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
High-resolution structural analyses were performed for NADH-cytochrome b5 reductase (b5R) and cytochrome b5 (b5) found in mammalian endoplasmic reticulum. The b5R and b5 redox system is involved in some metabolic processes including lipid synthesis. We have successfully determined high-resolution neutron and X-ray crystal structures for several redox states of b5R. The high-resolution structure analyses provide insights for understanding molecular mechanisms of the hydrogen movement that has important roles in the b5R redox cycle. Additionally, we could obtain high quality crystals of b5 with large volumes. Therefore, we will be able to perform high-resolution neutron structure analyses to visualize hydrogen atoms that have key roles in the b5 redox reaction.
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