Elucidation of functional regulatory mechanism of human globin protein that acts as a regulator of G protein
| Project/Area Number |
17K07329
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WAKASUGI KEISUKE 東京大学, 大学院総合文化研究科, 准教授 (20322167)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 蛋白質 / 蛋白質間相互作用 / 蛋白質工学 / 酸化ストレス / 細胞保護 / 生体分子 / シグナル伝達 / ストレス |
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| Outline of Final Research Achievements |
Neuroglobin (Ngb), which is expressed in human nervous system, protects neuronal cells against oxidative stress-induced cell death and induces neurite outgrowth at normal oxygen levels. In this study, we investigated functional characterization of human Ngb by protein engineering and analyzed the protein-protein interactions of Ngb. We clarified crucial amino acid residues for physiological functions of Ngb and for protein-protein interactions of Ngb. Furthermore, we identified a new protein that interacts with Ngb.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、脳卒中、脳梗塞など酸化ストレスに伴う神経細胞死を防ぎ、さらに神経再生を促す蛋白質であるニューログロビン(Ngb)の作用メカニズムがより詳細に明らかになった。また、Ngbが関わる蛋白質―蛋白質間相互作用についてもアミノ酸レベルで理解できるようになった。これらの研究成果は、脳卒中や脳梗塞のみならず、酸化ストレスに伴う他の疾患である癌、心筋梗塞、神経変性疾患などの治療薬開発にも役立つものと期待される。
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Report
(4 results)
Research Products
(19 results)
