| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We found molecular chaperone HSPA8 as a novel partner for ubiquitin-like protein MNSFβ. HSPA8 might promote the folding of the highly aggregable MNSFβ. Indeed, knockdown of HSPA8 significantly reduced the expression of MNSFβ in macrophage cell line Raw264.7 cells. We next examined the effect of HSPA8 on MNSFβ disaggregation. Although MNSFβ formed insoluble aggregation in vitro, HSPA8 significantly inhibited the aggregation of MNSFβ. Because HSPA8 has been reported to migrate from cytoplasm to nucleus during stress, we investigated the intracellular localization of MNSFβ. MNSFβ-GFP fusion protein localizes in the nucleus in unstimulated Hela cells, yet located to the cytoplasm by actinomycin D, a transcription inhibitor. MNSFβ siRNA increased the level of p53 expression in Raw264.7 cells under nutrient starvation conditions. Collectively, MNSFβ regulates cell proliferation through nucleolus stress response.
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