Structure-fuction relationship of specific DNA recognition machinery
| Project/Area Number |
17K07338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Science, Yamaguchi (2019)
Kyushu University (2017-2018) |
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Principal Investigator |
Kawakami Hironori 山陽小野田市立山口東京理科大学, 薬学部, 准教授 (50403952)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 染色体複製 / ORC / 機能構造解析 / DNA結合 / 高次複合体形成 / Cdc6 / 分子生物学 / 複製 |
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| Outline of Final Research Achievements |
The origin recognition complex, an initiator of eukaryotic DNA replication, was analyzed in vitro, in vivo, and in silico. The eukaryotic origin sensor motif in ORC, which was originally identified by our group, was shown to have a novel function. Another ORC-related protein was also analyzed. Based on these findings, possible models regarding DNA recognition processes were proposed.
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| Academic Significance and Societal Importance of the Research Achievements |
種々の蛋白質が染色体DNAの特定領域を走査探索・認識して高次複合体を形成することは、細胞内の普遍的プロセスである。染色体上の複製起点DNAに作られる高次複合体は、細胞周期制御の要であり、この形成過程が破綻すると、がん化や染色体異常の原因となる。本研究により、蛋白質とDNAとの結合メカニズムという根本的問題の一端が理解できたことに加え、高次複合体構築原理と機能分担、ならびにその普遍性が明らかとなった。関連分野における斬新なコンセプトを提示できたことに加え、がんの理解やORCを標的とした創薬の可能性が期待できる。
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Report
(4 results)
Research Products
(37 results)
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[Presentation] DNA binding of replication initiator in budding yeast2019
Author(s)
Hironori Kawakami, Ryuya Muraoka, Takuya Kurihara, Takeaki Chichibu, Shota Kanamoto, Kenta Kawabata, Eiji Ohashi, Toshiki Tsurimoto, Tsutomu Katayama
Organizer
The 42nd Annual Meeting of the Molecular Biology Society of Japan, workshop 2PW-04 New Frontier in studies of DNA replication (organizers: Hisao Masai and Hiroyuki Araki)
Related Report
Int'l Joint Research
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[Presentation] Dynamic mechanisms of higher-order complexes for replication initiation and regulation of the E. coli genome2019
Author(s)
Tsutomu Katayama, Kenya Miyoshi, Chihiro Hayashi, Ryusei Yoshida, Ryo Sugiyama, Ryuji Sakai, Yukari Sakiyama, Kazutoshi Kasho, Hironori Kawakami, Shogo Ozaki, Mariko Nagat
Organizer
The 92nd annual meeting of the Japanese Biochemical Society, Symposium #1S13a Flexibility of genome maintenance machineries on diverse genome structures (organizers: Asako Furukohri and Hisao Masai)
Related Report
Int'l Joint Research / Invited
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