Organelle contact site-mediated control of Golgi functions
| Project/Area Number |
17K07348
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
Wakana Yuichi 東京薬科大学, 生命科学部, 助教 (90635187)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ゴルジ体 / 小胞体 / タンパク質分泌 / オルガネラ膜接触部位 / コレステロール / オルガネラ膜接触 / 輸送小胞 / 小胞輸送 |
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| Outline of Final Research Achievements |
We have previously reported that transport of cholesterol and ceramide at endoplasmic reticulum (ER)-Golgi contact sites is important for biogenesis of the transport carriers called CARTS at the Golgi complex and protein secretion. Here, we report that the ER cholesterol sensor protein SCAP promotes the biogenesis of CARTS via ER-Golgi contact sites in a cholesterol-dependent manner. Also, an analysis with a cell line where ER-Golgi contact sites are visualized demonstrated that CARTS are formed at the sites immediately adjacent to the contact sites, suggesting the possibility that ER-Golgi contacts sites are important for determining the position and timing of CARTS biogenesis at the Golgi complex.
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| Academic Significance and Societal Importance of the Research Achievements |
SCAPは、コレステロール欠乏時に膜結合型転写因子であるSREBPを活性化し、コレステロールの生合成・取り込みを促進することが広く知られている。本研究成果は、十分量のコレステロールの存在下におけるSCAPの新たな機能を示すものである。コレステロールは、膜構造の流動性を司ることで、その機能発現に重要な役割を果たす脂質であるが、今回、タンパク質分泌を司るシグナル分子としての新たな側面を持つ可能性が示唆された。本研究成果は今後、タンパク質分泌異常が引き起こす様々な疾患の治療薬開発の糸口となることが期待される。
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] MT1-MMP recruits the ER-Golgi SNARE Bet1 for efficient MT1-MMP transport to the plasma membrane.2019
Author(s)
Miyagawa, T.,* Hasegawa, K.,* Aoki, Y.,* Watanabe, T.,* Otagiri, Y., Arasaki, K., Wakana, Y., Asano, K., Tanaka, M., Yamaguchi, H., Tagaya, M. and Inoue, H. (*, These authors contributed equally to this work.)
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Journal Title
J. Cell Biol.
Volume: 218
Issue: 10
Pages: 3355-3371
DOI
Related Report
Peer Reviewed / Open Access
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