| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We have previously reported that transport of cholesterol and ceramide at endoplasmic reticulum (ER)-Golgi contact sites is important for biogenesis of the transport carriers called CARTS at the Golgi complex and protein secretion. Here, we report that the ER cholesterol sensor protein SCAP promotes the biogenesis of CARTS via ER-Golgi contact sites in a cholesterol-dependent manner. Also, an analysis with a cell line where ER-Golgi contact sites are visualized demonstrated that CARTS are formed at the sites immediately adjacent to the contact sites, suggesting the possibility that ER-Golgi contacts sites are important for determining the position and timing of CARTS biogenesis at the Golgi complex.
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