| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, we used CRISPR knockout library to search for novel factors affecting glycolipid transport. We succeeded in identifying genes involved in glycolipid metabolisms with high coverage. We succeeded in identifying SYS1, a multi-spanning transmembrane protein localized in the Golgi apparatus, in Vero cells. Knockout of SYS1 gene reduced Gb3 and instead remarkably increased GlcCer. The SYS1 knockout cells also showed a change in the morphology of the Golgi apparatus from an elongated laminar to an aggregated form, and dot-like structures of a trans-Golgi network marker TGN46 around the Golgi apparatus. This indicates that SYS1 regulates glycan biosynthesis through its involvement in the Golgi apparatus and trans-Golgi network trafficking.
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