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Correlation between strcuture and function of dynamic network protein

Research Project

Project/Area Number 17K07361
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

Inoue Rintaro  京都大学, 複合原子力科学研究所, 准教授 (80563840)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords小角中性子散乱 / アルファークリスタリン / 重水素化 / クリスタリン / 中性子散乱 / アルファクリスタリン
Outline of Final Research Achievements

In this work, we have studied the relationship between the structure and function of alpha -crystallin through the complementary use of analytical ultacentrifuge and deuteration- assisted neutron scattering. In the case of alpha-crystallin hetro-oligomer that consists of alpha A and alpha B subunit, difference of exchange rate between alpha A and alpha B subunit was experimentally clarified. As for the subunit exchange rate under semi-concentrated system, its exchange rate was same as that of dilute one. From the analytical ultacentrifuge measurements, the coexistence of monomer and oligomer was observed regardless of protein concentration. Then, we assumed a model monomer association/disscociation model and succeeded to reproduce the experimental results with this model. Finally, it was concluded that such monomer was responsible for its function.

Academic Significance and Societal Importance of the Research Achievements

近年では、明確な四次構造を持たないタンパク質こそが高等生物の機能を司っていると考えられているが、その機能と構造を結びつける手法が十分には確立していなかった。そこで、本研究課題で培った重水素化支援小角中性子散乱と分析超遠心法の組み合わせは非常に動的な構造を有するタンパク質の特徴づける非常に有効な手法であることが明らかとなった。本手法は他のsmall heat shock proteinのみならずより動的な構造を有する天然変性タンパク質にも適用できると期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (20 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 5 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Int'l Joint Research] ANSTO(オーストラリア)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] ANSTO(オーストラリア)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Supramolecular tholos-like architecture constituted by archaeal proteins without functional annotation.2020

    • Author(s)
      Maho Yagi-Utsumi,Arunima Sikdar,Chihong Song,Jimin Park,Rintaro Inoue,Hiroki Watanabe,Raymond N Burton-Smith,Toshiya Kozai,Tatsuya Suzuki,Atsuji Kodama,Kentaro Ishii,Hirokazu Yagi,Tadashi Satoh,Susumu Uchiyama,Takayuki Uchihashi,Keehyoung Joo,Jooyoung Lee,Masaaki Sugiyama,Kazuyoshi Murata,Koichi Kato
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1540-1540

    • DOI

      10.1038/s41598-020-58371-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic Views of the Fc Region of Immunoglobulin G Provided by Experimental and Computational Observations2019

    • Author(s)
      Yanaka Saeko、Yogo Rina、Inoue Rintaro、Sugiyama Masaaki、Itoh Satoru G.、Okumura Hisashi、Miyanoiri Yohei、Yagi Hirokazu、Satoh Tadashi、Yamaguchi Takumi、Kato Koichi
    • Journal Title

      Antibodies

      Volume: 8 Issue: 3 Pages: 39-39

    • DOI

      10.3390/antib8030039

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural Insights into the Inhibition of Amyloid Fibril Formation by Fibrinogen via Interaction with Prefibrillar Intermediates2019

    • Author(s)
      Yamamoto Naoki、Akai Taiki、Inoue Rintaro、Sugiyama Masaaki、Tamura Atsuo、Chatani Eri
    • Journal Title

      Biochemistry

      Volume: 58 Issue: 24 Pages: 2769-2781

    • DOI

      10.1021/acs.biochem.9b00439

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Newly developed Laboratory-based Size exclusion chromatography Small-angle x-ray scattering System (La-SSS)2019

    • Author(s)
      R. Inoue, T. Nakagawa, K. Morishima, N. Sato, A. Okuda, R. Urade, R. Yogo, S. Yanaka, M. Yagi-Utsumi, K. Kato, K. Omoto, K. Ito, M. Sugiyama
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41598-019-48911-w

    • NAID

      120006799252

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Asp 58 modulates lens αA-crystallin oligomer formation and chaperone function2018

    • Author(s)
      Takata Takumi、Nakamura-Hirota Tooru、Inoue Rintaro、Morishima Ken、Sato Nobuhiro、Sugiyama Masaaki、Fujii Noriko
    • Journal Title

      The FEBS Journal

      Volume: in press Issue: 12 Pages: 2263-2277

    • DOI

      10.1111/febs.14475

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Purification and structural characterization of water‐soluble menaquinone‐7 produced by Bacillus subtilis natto2018

    • Author(s)
      Chatake Toshiyuki、Yanagisawa Yasuhide、Inoue Rintaro、Sugiyama Masaaki、Matsuo Tatsuhito、Fujiwara Satoru、Ohsugi Tadanori、Sumi Hiroyuki
    • Journal Title

      Journal of Food Biochemistry

      Volume: 42 Issue: 6 Pages: e12630-e12630

    • DOI

      10.1111/jfbc.12630

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Experimental investigation of the glass transition of polystyrene thin films in a broad frequency range2018

    • Author(s)
      Inoue Rintaro、Kanaya Toshiji、Yamada Takeshi、Shibata Kaoru、Fukao Koji
    • Journal Title

      Physical Review E

      Volume: 97 Issue: 1 Pages: 012501-012501

    • DOI

      10.1103/physreve.97.012501

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation2018

    • Author(s)
      M. Yagi-Utsumi, A. Sikdar, T. Kozai, R. Inoue, M. Sugiyama, T. Uchihashi, H. Yagi, T. Satoh, and K. Kato
    • Journal Title

      Protein Engineering, Design, and Selection

      Volume: 31 Issue: 1 Pages: 29-36

    • DOI

      10.1093/protein/gzx066

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quasielastic neutron scattering study of microscopic dynamics in polybutadiene reinforced with an unsaturated carboxylate2017

    • Author(s)
      Mashita Ryo、Inoue Rintaro、Tominaga Taiki、Shibata Kaoru、Kishimoto Hiroyuki、Kanaya Toshiji
    • Journal Title

      Soft Matter

      Volume: 13 Issue: 43 Pages: 7862-7869

    • DOI

      10.1039/c7sm01262d

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of heteromolecular binding sites in transcription factors Sp1 and TAF4 using high-resolution nuclear magnetic resonance spectroscopy2017

    • Author(s)
      Hibino Emi、Inoue Rintaro、Sugiyama Masaaki、Kuwahara Jun、Matsuzaki Katsumi、Hoshino Masaru
    • Journal Title

      Protein Science

      Volume: 26 Issue: 11 Pages: 2280-2290

    • DOI

      10.1002/pro.3287

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Subunit dynamics in a-crystallin through deuteration-assisted small-angle neutron scattering2019

    • Author(s)
      R. Inoue, Y. Sakamaki, K. Wood1, K. Morishima, T. Takumi, N. Sato, A. Okuda, R. Urade, N. Fujii, M. Sugiyama
    • Organizer
      J-PARC symposium 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Subunit dynamics of a-Crystallin under crowding environment2018

    • Author(s)
      Rintaro Inoue, Yusuke Sakamaki, Takumi Takata, Ken Morishima, Nobuhiro Sato, Kathleen Wood, Noriko Fujii, Masaaki Sugiyama
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 準濃厚環境下におけるa-クリスタリンの動態2018

    • Author(s)
      井上倫太郎、酒巻裕介、守島健、高田匠、佐藤信浩、Kathleen Wood, 裏出令子、藤井紀子、杉山正明
    • Organizer
      日本中性子科学会 第18回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Laboratory based SEC SAXS SYSTEM (La-SSS)2018

    • Author(s)
      Rintaro Inoue, Ken Morishima, Nobuhiro Sato, Masaaki Sugiyama, Tatsuo Nakagawa
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Laboratory based SEC SAXS SYSTEM (La-SSS)2018

    • Author(s)
      Rintaro Inoue, Ken Morishima, Nobuhiro Sato, Masaaki Sugiyama, Tatsuo Nakagawa
    • Organizer
      SAS2018
    • Related Report
      2018 Research-status Report
  • [Presentation] Protein internal dynamics as revealed by neutron spin echo and MD simulation2018

    • Author(s)
      R.Inoue
    • Organizer
      The 8th Taiwan-Japan Joint Meeting on Neutron and X-ray Scattering
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Protein dynamics as revealed by neutron spin echo (NSE) and MD simulation2017

    • Author(s)
      R.Inoue, M. Sugiyama, T. Oroguchi, T.Oda, M. Sato, B. Farago, A. Martel, L. Porcar
    • Organizer
      第55回生物物理学会年会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 混雑環境下におけるα-crystallinの構造及びダイナミクス2017

    • Author(s)
      酒巻裕介、井上倫太郎、守島健、佐藤信浩、藤井紀子、杉山正明
    • Organizer
      日本中性子科学会 第17回年会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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