Study on the thermodynamics and regulation of amyloid formation using calorimetry
Project/Area Number |
17K07362
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | Osaka University |
Principal Investigator |
李 映昊 大阪大学, たんぱく質研究所, 講師 (70589431)
|
Project Period (FY) |
2017-04-01 – 2019-03-31
|
Project Status |
Discontinued (Fiscal Year 2018)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | Amyloid fibril / Calorimetry / Energy landscape / Kinetics / Thermodynamics / Protein aggregation / Protein misfolding / Misfolding and disease / Amyloid |
Outline of Annual Research Achievements |
In order to study thermodynamics of protein misfolding-induced aggregation and establish a new assay, isothermal titration calorimetry (ITC) was performed on amyloidogenesis of several amyloidogenic proteins and peptides. I revealed that amyloid formation resulited in exothermic heat after the lag time, and obtained thermodynamic parameters such as the change in enthalpy using in-house algorithms. I further challenged to elucidate the kinetic property of amyloid formation, and could calculate the rate constant of nucleation and elongation of amyloid fibrillation. Energy landscape built using these parameters revealed the polymorphic amyloidogenesis of b2m.ITC-based assay provides a powerful method for the study of the thermodynamics, kinetics, and mechanism of protein aggregation.
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Report
(2 results)
Research Products
(24 results)
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[Journal Article] Graphene quantum dots prevent α-synucleinopathy in Parkinson’s disease2018
Author(s)
Kim Donghoon、Yoo Je Min、Hwang Heehong、Lee Junghee、Lee Su Hyun、Yun Seung Pil、Park Myung Jin、Lee MinJun、Choi Seulah、Kwon Sang Ho、Lee Saebom、Kwon Seung-Hwan、Kim Sangjune、Park Yong Joo、Kinoshita Misaki、Lee Young-Ho、Shin Seokmin、Paik Seung R.、Lee Sung Joong、Lee Seulki、Hong Byung Hee、Ko Han Seok
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Journal Title
Nature Nanotechnology
Volume: -
Issue: 9
Pages: 812-818
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Stability and subunit assembly of nylon hydrolase: structural basis of correct subunit assembly, aggregation, and intracellular degradation of the precursor protein2018
Author(s)
Seiji Negoro, Naoki Shibata, Young-Ho Lee, Ikki Takehara, Ryo Kinugasa, Keisuke Nagai, Yusuke Tanaka, Dai-ichiro Kato, Masahiro Takeo, Yuji Goto and Yoshiki Higuchi
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 9725-9725
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra.2018
Author(s)
Micsonai, A., Wien, F., Bulyáki, E., Kun, J., Moussong, E., Lee, Y.-H., Goto, Y., Réfrégiers, M., Kardos, J.
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Journal Title
Nucleic Acids Research
Volume: 46 (W1)
Issue: W1
Pages: 315-322
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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